Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613144135_jinj CTCCCAAAGATGGCAAGGTCTAACCATACATG(+AGGACA)GATCCTCTGGCTTTGGTTTCTGCGTTAAGTA NGG 10 8 17 8 24 0 100 5 10
    240613144133_9agj AGTATTACTTAACGCAGAAACCAAAGCCAGAG(+AGGACA)GATCCATGTATGGTTAGACCTTGCCATCTTT NGG 10 8 17 8 24 0 100 5 10
    240613144132_42j2 ATTTGTTTTATATGAAATAATTTATTTTTCAG(+AGGACA)GTACCATTTTTTTTTTCATTTTAACTTTGTT NGG 10 8 17 8 24 0 100 5 10
    240613144131_pygp GTAAAAACAAAGTTAAAATGAAAAAAAAAATG(+AGGACA)GTACCTGAAAAATAAATTATTTCATATAAAA NGG 10 8 17 8 24 0 100 5 10
    240613144129_om6g CCTACTGGGACCCGAGGTAACTCCTGGGGAGG(+AGGACA)GATCCCTGGGGTTCATCATACTCAAGGGGTC NGG 10 8 17 8 24 0 100 5 10
    240613144126_bdfl TTCCTGACCCCTTGAGTATGATGAACCCCAGG(+AGGACA)GATCCCTCCCCAGGAGTTACCTCGGGTCCCA NGG 10 8 17 8 24 0 100 5 10
    240613144123_fxiu GTCCACTGAGGTGGGGAGAGTGTGCTGGGGGG(+AGGACA)GATCCAGATGCTAGAGCCACACACAAAGGGG NGG 10 8 17 8 24 0 100 5 10
    240613144122_9nom CATTACCCCTTTGTGTGTGGCTCTAGCATCTG(+AGGACA)GATCCCCCCCAGCACACTCTCCCCACCTCAG NGG 10 8 17 8 24 0 100 5 10
    240613144120_pubj TCGGTCGGACATGGAGTAGTTTGCTTTCCTGG(+AGGACA)GTACCCCTTGCTACAGCACCGTTGTCCTGTG NGG 10 8 17 8 24 0 100 5 10
    240613144117_mgv7 GTGCACACAGGACAACGGTGCTGTAGCAAGGG(+AGGACA)GTACCCAGGAAAGCAAACTACTCCATGTCCG NGG 10 8 17 8 24 0 100 5 10