Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613141857_zzdf GTGTTTCCTGTCGCTGTGGTTACATGGAGGTG(+AGGACA)GATCCTTCAGCTTGATTTCTGGCCATTTCCC NGG 10 8 17 8 24 0 100 5 10
    240613141855_1fu2 ATCATCACCTTACAGATGTGCCACGCGAAGCG(+AGGACA)GATCCATTCACCAATGCCTGTGTTCTGTGAC NGG 10 8 17 8 24 0 100 5 10
    240613141854_ul1z TTCTGGTCACAGAACACAGGCATTGGTGAATG(+AGGACA)GATCCGCTTCGCGTGGCACATCTGTAAGGTG NGG 10 8 17 8 24 0 100 5 10
    240613141853_epy1 AGGGTACTTGTGGAGGCCAGAAGAGGGTATTG(+AGGACA)GATCCTCTGGGGCTGGAGTTATGGGCAGCTG NGG 10 8 17 8 24 0 100 5 10
    240613141851_os9s GCTTCCAGCTGCCCATAACTCCAGCCCCAGAG(+AGGACA)GATCCAATACCCTCTTCTGGCCTCCACAAGT NGG 10 8 17 8 24 0 100 5 10
    240613141849_838g TTTGGAGCCTTAGTATGTTTATTTTGTGGGAG(+AGGACA)GATCCGCACGCACACAGCTCACTGGAGGGAG NGG 10 8 17 8 24 0 100 5 10
    240613141848_bgcp GTCATCTCCCTCCAGTGAGCTGTGTGCGTGCG(+AGGACA)GATCCTCCCACAAAATAAACATACTAAGGCT NGG 10 8 17 8 24 0 100 5 10
    240613141846_e6yl GGTTTGGTTCTTTCTTAGTACTCACAGTGGGG(+AGGACA)GATCCTGTGGCTCTGATGCTGTCTTCTGACC NGG 10 8 17 8 24 0 100 5 10
    240613141844_xci8 ATGCAGGTCAGAAGACAGCATCAGAGCCACAG(+AGGACA)GATCCCCCACTGTGAGTACTAAGAAAGAACC NGG 10 8 17 8 24 0 100 5 10
    240613141842_ku1d AGCAATTTAGAGCACTGACTGCTCTTCCTCAG(+AGGACA)GATCCAGGTTTGACTCCCAGTAACCATATGA NGG 10 8 17 8 24 0 100 5 10