Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613141840_fkcr AAGTTTCATATGGTTACTGGGAGTCAAACCTG(+AGGACA)GATCCTGAGGAAGAGCAGTCAGTGCTCTAAA NGG 10 8 17 8 24 0 100 5 10
    240613141839_bnt0 TGCCTGTCACTTACTTACTGCTCGGTTGATAG(+AGGACA)GTACCACACAGCATCTCCATTTCTTCCAGGC NGG 10 8 17 8 24 0 100 5 10
    240613141838_392m GAAAGGCCTGGAAGAAATGGAGATGCTGTGTG(+AGGACA)GTACCTATCAACCGAGCAGTAAGTAAGTGAC NGG 10 8 17 8 24 0 100 5 10
    240613141836_cdks GCCACAGCTCAGGTCTTCTCCACACTCGTCAG(+AGGACA)GTACCTGTGCTATGGAGTTTCTGCCCGAGTC NGG 10 8 17 8 24 0 100 5 10
    240613141835_m68y TTGTTGACTCGGGCAGAAACTCCATAGCACAG(+AGGACA)GTACCTGACGAGTGTGGAGAAGACCTGAGCT NGG 10 8 17 8 24 0 100 5 10
    240613141834_vw85 CATGGATTTGGGGATATTTTCCAGCTAGAATG(+AGGACA)GATCCAGAATTGAATGGTGCTTAAATTGAGA NGG 10 8 17 8 24 0 100 5 10
    240613141832_ov1x TTATTTCTCAATTTAAGCACCATTCAATTCTG(+AGGACA)GATCCATTCTAGCTGGAAAATATCCCCAAAT NGG 10 8 17 8 24 0 100 5 10
    240613141831_subr GGTATTTTCAGCTGCAGTTCTCCTCCTCTCTG(+AGGACA)GATCCTGGCTTGGGGTGCTTTTCCAGCATCC NGG 10 8 17 8 24 0 100 5 10
    240613141829_viwu ACTGTGGATGCTGGAAAAGCACCCCAAGCCAG(+AGGACA)GATCCAGAGAGGAGGAGAACTGCAGCTGAAA NGG 10 8 17 8 24 0 100 5 10
    240613141828_jtkh CTATTAGGAGAGTAGATGTTAATGTGTTTTGG(+AGGACA)GTACCCATCTTTGCAACATGTCTGATCCACG NGG 10 8 17 8 24 0 100 5 10