Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613140420_xrmn TATTAATGATACTCTGTTATGCTTGCAGACAG(+AGGACA)GATCCTAGTATAGCTGTCCTCAGAGAGGCTC NGG 10 8 17 8 24 0 100 5 10
    240613140418_qmo9 TTACACGAGTCAGTTCTTTTCTCTCCACCATG(+AGGACA)GATCCCACTGATCAAACCTGGGTTATCAGCC NGG 10 8 17 8 24 0 100 5 10
    240613140416_a4uk TGCAAGGCTGATAACCCAGGTTTGATCAGTGG(+AGGACA)GATCCATGGTGGAGAGAAAAGAACTGACTCG NGG 10 8 17 8 24 0 100 5 10
    240613140414_70zh TTTAAGCCCACCTGGTCACCCATCAAAGTCCG(+AGGACA)GATCCTGTCTTTCCCCGTTTACCCTTCCCAC NGG 10 8 17 8 24 0 100 5 10
    240613140413_jipt GGAGGGTGGGAAGGGTAAACGGGGAAAGACAG(+AGGACA)GATCCGGACTTTGATGGGTGACCAGGTGGGC NGG 10 8 17 8 24 0 100 5 10
    240613140412_ls45 GCTTTATTCATTATTGACATTTGTAGTTGAGG(+AGGACA)GATCCGGGGGTTCAATTTATTTTGATTTTTT NGG 10 8 17 8 24 0 100 5 10
    240613140407_fu6b AAAAAAAAAAATCAAAATAAATTGAACCCCCG(+AGGACA)GATCCCTCAACTACAAATGTCAATAATGAAT NGG 10 8 17 8 24 0 100 5 10
    240613140406_8pun TGGCCACCACCTCCCCCGGACTCTACCTCAAG(+AGGACA)GATCCACCTCAAACCAAGCCTGACCCAGGCA NGG 10 8 17 8 24 0 100 5 10
    240613140405_e50j GGTGATGCCTGGGTCAGGCTTGGTTTGAGGTG(+AGGACA)GATCCTTGAGGTAGAGTCCGGGGGAGGTGGT NGG 10 8 17 8 24 0 100 5 10
    240613140403_t54q AAGGAGGCGACGCAGAGGCACTGGGGTACAGG(+AGGACA)GTACCAAATGAAAGAGAGAGAGTCTTGAGAA NGG 10 8 17 8 24 0 100 5 10