Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613135714_3j4j CTGGAGAGACATTCTTGCTTCACTGTCCTATG(+AGGACA)GTACCTGTATCTCCATTATTTTCTGGAAATT NGG 10 8 17 8 24 0 100 5 10
    240613135713_bm6x GTTTTAGTTTACAAAAGAAAAATCAATGATTG(+AGGACA)GTACCTTTTTTTAACACTCTCAGATTCCTGA NGG 10 8 17 8 24 0 100 5 10
    240613135712_ql3w CATATTCAGGAATCTGAGAGTGTTAAAAAAAG(+AGGACA)GTACCAATCATTGATTTTTCTTTTGTAAACT NGG 10 8 17 8 24 0 100 5 10
    240613135710_vjts TGAAGACAATAAATATTCCTTTGAGACCCGTG(+AGGACA)GATCCTTCTGCGGACCCTAATGACACAAAGT NGG 10 8 17 8 24 0 100 5 10
    240613135708_u3v8 GCTCCACTTTGTGTCATTAGGGTCCGCAGAAG(+AGGACA)GATCCACGGGTCTCAAAGGAATATTTATTGT NGG 10 8 17 8 24 0 100 5 10
    240613135707_o8l5 ACCGCTCTCAAAGTAGACGGCATCGCTGCGTG(+AGGACA)GATCCACGCCGCTCACGTAAAAGCGGCGACA NGG 10 8 17 8 24 0 100 5 10
    240613135706_5gyg GGGGTTGTCGCCGCTTTTACGTGAGCGGCGTG(+AGGACA)GATCCACGCAGCGATGCCGTCTACTTTGAGA NGG 10 8 17 8 24 0 100 5 10
    240613135704_mawv TCCTTTGCCTTATCTCATGGGGATGGAGGAGG(+AGGACA)GTACCCAGGTCTCTGTTGCTTTGACTGTCTC NGG 10 8 17 8 24 0 100 5 10
    240613135702_3yr1 TTATTGAGACAGTCAAAGCAACAGAGACCTGG(+AGGACA)GTACCCTCCTCCATCCCCATGAGATAAGGCA NGG 10 8 17 8 24 0 100 5 10
    240613135700_6fq8 AGTACCTGAGGGTCTGTAGGTTGCACACTTTG(+AGGACA)GTACCAGGTAACTTTTTTTTCTTTATAAGAA NGG 10 8 17 8 24 0 100 5 10