Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613135817_99o1 CCCCCAAAAAAGGGGGGGAGGAGGCATGGTTG(+AGGACA)GTACCCACCCACCTGCACTGGTGAGGGAAGT NGG 10 8 17 8 24 0 100 5 10
    240613135815_4rc2 GGGCAAGCCGGTTCTCTCCTTCCACCAGATGG(+AGGACA)GATCCCAGGGGATTGAATTCAGGTTATCTGG NGG 10 8 17 8 24 0 100 5 10
    240613135812_2jf5 AAATCCCAGATAACCTGAATTCAATCCCCTGG(+AGGACA)GATCCCATCTGGTGGAAGGAGAGAACCGGCT NGG 10 8 17 8 24 0 100 5 10
    240613135811_rjd6 CCTGTGACCCTGCATCCCATGGGTCCTGTGAG(+AGGACA)GTACCCGAGCCCCCTTTCTAACTCCCACCTC NGG 10 8 17 8 24 0 100 5 10
    240613135809_vzy1 TTGGGGAGGTGGGAGTTAGAAAGGGGGCTCGG(+AGGACA)GTACCTCACAGGACCCATGGGATGCAGGGTC NGG 10 8 17 8 24 0 100 5 10
    240613135808_bzxb CTTTGTCCACTGCAGTAAATTCCACTGTGTGG(+AGGACA)GATCCCCACAGACTTCTGGACAAGGTGGCTT NGG 10 8 17 8 24 0 100 5 10
    240613135806_teu3 TGGGGAAGCCACCTTGTCCAGAAGTCTGTGGG(+AGGACA)GATCCCACACAGTGGAATTTACTGCAGTGGA NGG 10 8 17 8 24 0 100 5 10
    240613135805_d3mr TGCCCCAGGCTCCAGAGCAAGTAGGACGTCAG(+AGGACA)GTACCTGCTCTCTTGCCTGGCTCTAGATTTT NGG 10 8 17 8 24 0 100 5 10
    240613135803_3uf2 AAATTAAAATCTAGAGCCAGGCAAGAGAGCAG(+AGGACA)GTACCTGACGTCCTACTTGCTCTGGAGCCTG NGG 10 8 17 8 24 0 100 5 10
    240613135802_bdo4 GCCAAGTGGGACGCATTGACTGGTCACACGTG(+AGGACA)GTACCTCTGGCCTTTCTTTTATTTATTTATT NGG 10 8 17 8 24 0 100 5 10