Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613135908_z1c2 TGGAAGGGGTGGGTGGGCCTCCAGGAAGGTGG(+AGGACA)GATCCAAGTGTCCTTTCTGGAGTTGATTCTA NGG 10 8 17 8 24 0 100 5 10
    240613135906_pa76 CTGAATGATGTCACCACAGCCAGGGCTCCAGG(+AGGACA)GTACCCCCACATCCAGAGCAGCTCACAGCTG NGG 10 8 17 8 24 0 100 5 10
    240613135904_8l67 AAAGGCAGCTGTGAGCTGCTCTGGATGTGGGG(+AGGACA)GTACCCTGGAGCCCTGGCTGTGGTGACATCA NGG 10 8 17 8 24 0 100 5 10
    240613135902_j8ui GTTTTATTAGAAAACAAGCTAGGTGGGGAAGG(+AGGACA)GATCCTTCCAAACCACCATGGAGGTTTGATA NGG 10 8 17 8 24 0 100 5 10
    240613135900_zloc TAAATTATCAAACCTCCATGGTGGTTTGGAAG(+AGGACA)GATCCCTTCCCCACCTAGCTTGTTTTCTAAT NGG 10 8 17 8 24 0 100 5 10
    240613135859_sf71 TTGTTCTAGAAGGCGCTCCTTTCAGGGTTGTG(+AGGACA)GTACCTGTAGGTTAGCAGACCCTCCTCCTTT NGG 10 8 17 8 24 0 100 5 10
    240613135858_75di GTGGGAAAGGAGGAGGGTCTGCTAACCTACAG(+AGGACA)GTACCACAACCCTGAAAGGAGCGCCTTCTAG NGG 10 8 17 8 24 0 100 5 10
    240613135856_hl7k CCTTCTACCAAGTGGGTAGTCCGTCCTGATAG(+AGGACA)GTACCTTTACCTGCTGAGCCATCTGAGTAGC NGG 10 8 17 8 24 0 100 5 10
    240613135855_ncvv GTGAGGCTACTCAGATGGCTCAGCAGGTAAAG(+AGGACA)GTACCTATCAGGACGGACTACCCACTTGGTA NGG 10 8 17 8 24 0 100 5 10
    240613135853_g3jm CAGTGGGTTGCTGCTCTCCAGGGATGTGGCTG(+AGGACA)GTACCCCAGGTTCTTCCAGGTCATTCTCCTG NGG 10 8 17 8 24 0 100 5 10