Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613135957_hwar GGCCTTTCCATTGCAGAGTTCCATCATTTATG(+AGGACA)GTACCAATGTAATCCATCTCCACCAGTCAAA NGG 10 8 17 8 24 0 100 5 10
    240613135954_6xgn AGGGTAAACAAGGGAGACTGCTGCAGCCCCTG(+AGGACA)GATCCCAAATGCCCATGTCTGAGCCACACGA NGG 10 8 17 8 24 0 100 5 10
    240613135953_v85u GAGCTTCGTGTGGCTCAGACATGGGCATTTGG(+AGGACA)GATCCAGGGGCTGCAGCAGTCTCCCTTGTTT NGG 10 8 17 8 24 0 100 5 10
    240613135950_h887 AGGGTAAACAAGGGAGACTGCTGCAGCCCCTG(+AGGACA)GATCCCAAATGCCCATGTCTGAGCCACACGA NGG 10 8 17 8 24 0 100 5 10
    240613135948_beox GAGCTTCGTGTGGCTCAGACATGGGCATTTGG(+AGGACA)GATCCAGGGGCTGCAGCAGTCTCCCTTGTTT NGG 10 8 17 8 24 0 100 5 10
    240613135947_10i1 CAAAGGCTCCAGTTCCTACAAGTTGGTAGAGG(+AGGACA)GATCCAGGTGTACTTTCAGTCACTGGAAGAA NGG 10 8 17 8 24 0 100 5 10
    240613135945_7dy6 TGTAATTCTTCCAGTGACTGAAAGTACACCTG(+AGGACA)GATCCCTCTACCAACTTGTAGGAACTGGAGC NGG 10 8 17 8 24 0 100 5 10
    240613135944_9p2d TGTCCGGGTCCCATGTGAGTGACTCTAGGAAG(+AGGACA)GATCCTAGGATTGTGAAGTGAACAGTAACAC NGG 10 8 17 8 24 0 100 5 10
    240613135942_1ona GACAAGTGTTACTGTTCACTTCACAATCCTAG(+AGGACA)GATCCTTCCTAGAGTCACTCACATGGGACCC NGG 10 8 17 8 24 0 100 5 10
    240613135940_a5ye ACACCGAGGAACCCCAGTTAGGAAGATCTGGG(+AGGACA)GTACCAGAGAATGCTTGCCCTTTCCTTCAGG NGG 10 8 17 8 24 0 100 5 10