Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613135939_v1ii GCCAACCTGAAGGAAAGGGCAAGCATTCTCTG(+AGGACA)GTACCCCAGATCTTCCTAACTGGGGTTCCTC NGG 10 8 17 8 24 0 100 5 10
    240613135937_3vqz CAGTGAGCATCCTGCCTGCCTATGCATTTTGG(+AGGACA)GTACCTCCCCTGTGACACCCACCCCCAGAGC NGG 10 8 17 8 24 0 100 5 10
    240613135935_c6bx ATACAGCTCTGGGGGTGGGTGTCACAGGGGAG(+AGGACA)GTACCCAAAATGCATAGGCAGGCAGGATGCT NGG 10 8 17 8 24 0 100 5 10
    240613135933_8gc4 CTTGTATACTGCAAGACAGGTCACACACCAAG(+AGGACA)GATCCTTCAAACACAAACGCTCCTCCCCTTC NGG 10 8 17 8 24 0 100 5 10
    240613135932_uzfh ATCAAGAAGGGGAGGAGCGTTTGTGTTTGAAG(+AGGACA)GATCCTTGGTGTGTGACCTGTCTTGCAGTAT NGG 10 8 17 8 24 0 100 5 10
    240613135931_dbbn TCAAGAGGCAAAGTCTCCACTTTCCTACACAG(+AGGACA)GATCCATCTTTTGTCTCAGCCAACTCTCAAT NGG 10 8 17 8 24 0 100 5 10
    240613135930_7ip5 CCTCCATTGAGAGTTGGCTGAGACAAAAGATG(+AGGACA)GATCCTGTGTAGGAAAGTGGAGACTTTGCCT NGG 10 8 17 8 24 0 100 5 10
    240613135928_c76r GGGAAGAACTGTTAAGTATCGCTAGGGTCAGG(+AGGACA)GATCCTGACACCTTGGTCAGCTACCAAACAC NGG 10 8 17 8 24 0 100 5 10
    240613135927_rtnf CAAGAGTGTTTGGTAGCTGACCAAGGTGTCAG(+AGGACA)GATCCCTGACCCTAGCGATACTTAACAGTTC NGG 10 8 17 8 24 0 100 5 10
    240613135925_a7pc GCTCCAGTGCCTACAGCTGCAGGGTGGCATTG(+AGGACA)GATCCTGTGACTCCCGGTCCTTCCTAAGTGA NGG 10 8 17 8 24 0 100 5 10