Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613135834_oi73 ATGTGGCTGGACATTTGAAGATAGGACACAGG(+AGGACA)GATCCACTCCTTATTTCTTCATCTCTTAAGA NGG 10 8 17 8 24 0 100 5 10
    240613135832_cab6 TGGGCTCCTGATTAATGAATTTCACAACAATG(+AGGACA)GTACCCAGGCAGGAGTCCCCGTGGCTGGACC NGG 10 8 17 8 24 0 100 5 10
    240613135830_0w8x TCAGAGGTCCAGCCACGGGGACTCCTGCCTGG(+AGGACA)GTACCATTGTTGTGAAATTCATTAATCAGGA NGG 10 8 17 8 24 0 100 5 10
    240613135828_rgvl CTCTAGATACAGCTGTGCCTGGAGGGCCACTG(+AGGACA)GATCCCCACCCGTTAGATCTCGAGGGCATCA NGG 10 8 17 8 24 0 100 5 10
    240613135826_x8yf AGACCTGATGCCCTCGAGATCTAACGGGTGGG(+AGGACA)GATCCAGTGGCCCTCCAGGCACAGCTGTATC NGG 10 8 17 8 24 0 100 5 10
    240613135825_p3g2 TCTGGACCCAAGGCTAGAATGTCTCCAGCTGG(+AGGACA)GATCCCTGAGTTGGGCAGGCCCAACCCTCCA NGG 10 8 17 8 24 0 100 5 10
    240613135823_c4r1 GCCACTGGAGGGTTGGGCCTGCCCAACTCAGG(+AGGACA)GATCCCAGCTGGAGACATTCTAGCCTTGGGT NGG 10 8 17 8 24 0 100 5 10
    240613135822_12xt AATCACATTCTCTGTTCAGCCGTTAATGCTGG(+AGGACA)GATCCATATTTAGTTTTATAAGCTTTTCCCT NGG 10 8 17 8 24 0 100 5 10
    240613135820_mvlw AAAACAGGGAAAAGCTTATAAAACTAAATATG(+AGGACA)GATCCCAGCATTAACGGCTGAACAGAGAATG NGG 10 8 17 8 24 0 100 5 10
    240613135818_6tsx TAGTCACTTCCCTCACCAGTGCAGGTGGGTGG(+AGGACA)GTACCAACCATGCCTCCTCCCCCCCTTTTTT NGG 10 8 17 8 24 0 100 5 10