Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613135924_mm69 TCCTTTCACTTAGGAAGGACCGGGAGTCACAG(+AGGACA)GATCCAATGCCACCCTGCAGCTGTAGGCACT NGG 10 8 17 8 24 0 100 5 10
    240613135922_5ge8 TTTAATTAAAGAGAATTCTTCCACTTCGTCGG(+AGGACA)GATCCGTCTTCTGCAATCAAAGTGGACTGCT NGG 10 8 17 8 24 0 100 5 10
    240613135921_5v0a CCTCAAGCAGTCCACTTTGATTGCAGAAGACG(+AGGACA)GATCCCGACGAAGTGGAAGAATTCTCTTTAA NGG 10 8 17 8 24 0 100 5 10
    240613135920_16yj TGTGCTGTCACCACTCCCCAATGTCCTGGGAG(+AGGACA)GTACCTCCAGTCAGAGGCAAAGAGGCCATGA NGG 10 8 17 8 24 0 100 5 10
    240613135918_9770 CTCTGTCATGGCCTCTTTGCCTCTGACTGGAG(+AGGACA)GTACCTCCCAGGACATTGGGGAGTGGTGACA NGG 10 8 17 8 24 0 100 5 10
    240613135917_s8bx TGTGGGGTGGGCTCTGGAGGCAGGCGACAGTG(+AGGACA)GTACCAGGCTGCCTACTGAGTGTCTATGGGG NGG 10 8 17 8 24 0 100 5 10
    240613135915_txu6 AGCGCCCCCATAGACACTCAGTAGGCAGCCTG(+AGGACA)GTACCACTGTCGCCTGCCTCCAGAGCCCACC NGG 10 8 17 8 24 0 100 5 10
    240613135914_w5fr AATTTATTTTAGTTTTTATTTCTCAATGAAAG(+AGGACA)GATCCCTATGAGGTAGTTTTTGCCCATCTAG NGG 10 8 17 8 24 0 100 5 10
    240613135912_i4lm AAGACCTAGATGGGCAAAAACTACCTCATAGG(+AGGACA)GATCCTTTCATTGAGAAATAAAAACTAAAAT NGG 10 8 17 8 24 0 100 5 10
    240613135910_rpex GGACCTAGAATCAACTCCAGAAAGGACACTTG(+AGGACA)GATCCCACCTTCCTGGAGGCCCACCCACCCC NGG 10 8 17 8 24 0 100 5 10