Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613134052_iqfj TTCTCTGCAGCACTCAGGGAGCCCGGTAAAGG(+AGGACA)GTACCTCCCGGAAGAATGTGGTTTTTTTTTA NGG 10 8 17 8 24 0 100 5 10
    240613134050_885b GGCACTTCAGAGAAAAACTAATGACAGCCTCG(+AGGACA)GTACCTCGAACCTCATCGTTGTCTTCTTTCT NGG 10 8 17 8 24 0 100 5 10
    240613134048_7mzj AGTTGAGAAAGAAGACAACGATGAGGTTCGAG(+AGGACA)GTACCGAGGCTGTCATTAGTTTTTCTCTGAA NGG 10 8 17 8 24 0 100 5 10
    240613134047_grqb GGTTTGGAGAAAGATCCACACGTCAAAAGGTG(+AGGACA)GTACCTGAATATGCACCAATTAGCAAACGAA NGG 10 8 17 8 24 0 100 5 10
    240613134046_oaf0 TTTGTTTCGTTTGCTAATTGGTGCATATTCAG(+AGGACA)GTACCACCTTTTGACGTGTGGATCTTTCTCC NGG 10 8 17 8 24 0 100 5 10
    240613134045_p66t AAAAGCCCCTATGGAAAGCAAATTCCTGATTG(+AGGACA)GTACCACCCTCGAATAGCTTATATCGTTAAA NGG 10 8 17 8 24 0 100 5 10
    240613134043_i35t CTCTATTTAACGATATAAGCTATTCGAGGGTG(+AGGACA)GTACCAATCAGGAATTTGCTTTCCATAGGGG NGG 10 8 17 8 24 0 100 5 10
    240613134042_kfzz GGGCCTGTTGTCCACGGAAGGCATTTGCTTAG(+AGGACA)GATCCCCTTTGCCCCTGGCTCGGCCAGATGA NGG 10 8 17 8 24 0 100 5 10
    240613134040_4ct1 ACCTTTCATCTGGCCGAGCCAGGGGCAAAGGG(+AGGACA)GATCCTAAGCAAATGCCTTCCGTGGACAACA NGG 10 8 17 8 24 0 100 5 10
    240613134039_wyqc TTACATGATAAGAAAGCTTGACACAAGCTGAG(+AGGACA)GTACCAAGAGGCTCCTGCCACCTGGTCCACG NGG 10 8 17 8 24 0 100 5 10