Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613134023_fur5 GTCATTTTATTGCTTTTATAAATAGACAAGGG(+AGGACA)GATCCTCATGCCCCGTCCTTTTGTGAAGCAG NGG 10 8 17 8 24 0 100 5 10
    240613134021_ure0 TGGGAATCATTGTCCAGGAGTAACTCCAGCAG(+AGGACA)GATCCCAGGTCTCTGCAGAAGCAGGAAGGTG NGG 10 8 17 8 24 0 100 5 10
    240613134019_wwh3 CAAGCCACCTTCCTGCTTCTGCAGAGACCTGG(+AGGACA)GATCCTGCTGGAGTTACTCCTGGACAATGAT NGG 10 8 17 8 24 0 100 5 10
    240613134018_okca CTCTTCCCAAAGAGCCACTTACCTCTGGACAG(+AGGACA)GTACCCTGCGTCTGGTTCACGGTGACGATCA NGG 10 8 17 8 24 0 100 5 10
    240613134016_dkf5 CAACATGATCGTCACCGTGAACCAGACGCAGG(+AGGACA)GTACCTGTCCAGAGGTAAGTGGCTCTTTGGG NGG 10 8 17 8 24 0 100 5 10
    240613134015_q269 TTGTATGCTGACAACTTTAATAATGTAAGGCG(+AGGACA)GATCCGGCTCACAAGCCGGGAGGCAGCACAG NGG 10 8 17 8 24 0 100 5 10
    240613134013_k571 CTTGTCTGTGCTGCCTCCCGGCTTGTGAGCCG(+AGGACA)GATCCGCCTTACATTATTAAAGTTGTCAGCA NGG 10 8 17 8 24 0 100 5 10
    240613134012_9jyt GCCAAGTGCTGCTCTGCAGTCCGTGAAGCACG(+AGGACA)GTACCCGCATCCGGACCATCATGACTATAGG NGG 10 8 17 8 24 0 100 5 10
    240613134010_1a5l CACTCCCTATAGTCATGATGGTCCGGATGCGG(+AGGACA)GTACCGTGCTTCACGGACTGCAGAGCAGCAC NGG 10 8 17 8 24 0 100 5 10
    240613134008_731g GGTACAGCTGGATGCTGCAGAGGAGGGACTGG(+AGGACA)GTACCATGATGGCTCCCCTAGGGAGGCAGCA NGG 10 8 17 8 24 0 100 5 10