Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613133935_olqa TGTTTCAAAGTTACATGTGTCAAAAATTCCAG(+AGGACA)GTACCTTTTATTTTCATCATATCTATGGTCC NGG 10 8 17 8 24 0 100 5 10
    240613133934_dvsu ACTGCTAGGCTTTTTGATGCAAGTTGGATTGG(+AGGACA)GATCCTTCCAGGTCTCTTCTTATACATACAC NGG 10 8 17 8 24 0 100 5 10
    240613133932_9ot9 CTTGTGTGTATGTATAAGAAGAGACCTGGAAG(+AGGACA)GATCCCAATCCAACTTGCATCAAAAAGCCTA NGG 10 8 17 8 24 0 100 5 10
    240613133931_dgvy CTGGGGGCCCATAGGTATGATGAAAATAAAAG(+AGGACA)GTACCTGGAATTTTTGACACATGTAACTTTG NGG 10 8 17 8 24 0 100 5 10
    240613133929_pg4i TGTTTCAAAGTTACATGTGTCAAAAATTCCAG(+AGGACA)GTACCTTTTATTTTCATCATACCTATGGGCC NGG 10 8 17 8 24 0 100 5 10
    240613133928_91b9 ACTGCTAGGCTTTTTGATGCAAGTTGGATTGG(+AGGACA)GATCCTTCCAGGTCTCTTCTTACACATACAC NGG 10 8 17 8 24 0 100 5 10
    240613133926_ognt CTTGTGTGTATGTGTAAGAAGAGACCTGGAAG(+AGGACA)GATCCCAATCCAACTTGCATCAAAAAGCCTA NGG 10 8 17 8 24 0 100 5 10
    240613133925_m1j7 CTGTGGAAGAGTTTCTATGTGAGATGAGGATG(+AGGACA)GATCCCTCAGAGTACACACCCATCTCATGGC NGG 10 8 17 8 24 0 100 5 10
    240613133924_g9vu AGCAAGCCATGAGATGGGTGTGTACTCTGAGG(+AGGACA)GATCCATCCTCATCTCACATAGAAACTCTTC NGG 10 8 17 8 24 0 100 5 10
    240613133922_wnsv ACTGTTACACGTATTGATGTTGGGTAGCTTGG(+AGGACA)GATCCTTCTAGATCTCTGATGCAAGAAACCC NGG 10 8 17 8 24 0 100 5 10