Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613133921_int2 AGTCTGGGTTTCTTGCATCAGAGATCTAGAAG(+AGGACA)GATCCCAAGCTACCCAACATCAATACGTGTA NGG 10 8 17 8 24 0 100 5 10
    240613133920_8qck GTACAGTGGATCTGAGCGGCAGGGGATTATTG(+AGGACA)GTACCATCTGCTCTTCGGGCCCAACGGGGTA NGG 10 8 17 8 24 0 100 5 10
    240613133918_dyvo GGGACTACCCCGTTGGGCCCGAAGAGCAGATG(+AGGACA)GTACCAATAATCCCCTGCCGCTCAGATCCAC NGG 10 8 17 8 24 0 100 5 10
    240613133915_9c7l TGCCTGGTTCTAGGCCCCTTCCTCTACCCCAG(+AGGACA)GTACCCTCAGCCTAGGGGAGCAGGCTCAGGT NGG 10 8 17 8 24 0 100 5 10
    240613133913_4hfn CTTCCACCTGAGCCTGCTCCCCTAGGCTGAGG(+AGGACA)GTACCTGGGGTAGAGGAAGGGGCCTAGAACC NGG 10 8 17 8 24 0 100 5 10
    240613133912_080u CCCCCGGGAACCCCCACCCCCACCCCCGGCAG(+AGGACA)GATCCCTCCATGCAGGAGAAAGCCAACCAGA NGG 10 8 17 8 24 0 100 5 10
    240613133910_3nt2 GGGCCTCTGGTTGGCTTTCTCCTGCATGGAGG(+AGGACA)GATCCTGCCGGGGGTGGGGGTGGGGGTTCCC NGG 10 8 17 8 24 0 100 5 10
    240613133907_xpok TGTCGCCCAGGGCTGTAAGCTGAGACCCCTGG(+AGGACA)GATCCCTGTCTAAGCTAGATGCCCCTTCCCT NGG 10 8 17 8 24 0 100 5 10
    240613133905_oq74 AATGGAGGGAAGGGGCATCTAGCTTAGACAGG(+AGGACA)GATCCCAGGGGTCTCAGCTTACAGCCCTGGG NGG 10 8 17 8 24 0 100 5 10
    240613133903_xn1q CTGGGGCTTATGCTTGGCTGTGGGCCTGGTGG(+AGGACA)GATCCGGGGCACACATTCTCCTAGTGCTGGT NGG 10 8 17 8 24 0 100 5 10