Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613133804_jbkt CAAGTGGACTGGGGCATGCCACCCTTATGGGG(+AGGACA)GTACCAAGTGCCCCTGCTGAGGGAGGCTGGC NGG 10 8 17 8 24 0 100 5 10
    240613133803_208g AAAAGAGGGGGGTGGGGGAGCTCCATGGGGTG(+AGGACA)GATCCCAGGCTGGCAGCAGGAAGGTGCTGGA NGG 10 8 17 8 24 0 100 5 10
    240613133801_jjxb GGCCTTCCAGCACCTTCCTGCTGCCAGCCTGG(+AGGACA)GATCCACCCCATGGAGCTCCCCCACCCCCCT NGG 10 8 17 8 24 0 100 5 10
    240613133759_zvta TAAAAAGAAACTTATGATTTATTAGAGTACAG(+AGGACA)GATCCAAAACACATTTTTAGAAAAATATAAA NGG 10 8 17 8 24 0 100 5 10
    240613133758_m695 GGCACTTTATATTTTTCTAAAAATGTGTTTTG(+AGGACA)GATCCTGTACTCTAATAAATCATAAGTTTCT NGG 10 8 17 8 24 0 100 5 10
    240613133756_toog TCCCTTGGTCTTTACTCTCTACCACAGACCCG(+AGGACA)GTACCAGGTCTTGCCCACTTAGCTTAAGAAT NGG 10 8 17 8 24 0 100 5 10
    240613133754_4nzz CACGGATTCTTAAGCTAAGTGGGCAAGACCTG(+AGGACA)GTACCGGGTCTGTGGTAGAGAGTAAAGACCA NGG 10 8 17 8 24 0 100 5 10
    240613133752_7e0y TGAGGCAATTGGTTTGCCCTGCTGGGATATGG(+AGGACA)GATCCAGGCTTCGTATGCATGCTAGGCGAAC NGG 10 8 17 8 24 0 100 5 10
    240613133750_9aju GTAGAGTTCGCCTAGCATGCATACGAAGCCTG(+AGGACA)GATCCCATATCCCAGCAGGGCAAACCAATTG NGG 10 8 17 8 24 0 100 5 10
    240613133748_3p1r GGGCAGCCACCCCATATCCCATGGGGCGGGAG(+AGGACA)GATCCAGTTAGGAGCTGAAAACCCTGCTGGA NGG 10 8 17 8 24 0 100 5 10