Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613134015_q269 TTGTATGCTGACAACTTTAATAATGTAAGGCG(+AGGACA)GATCCGGCTCACAAGCCGGGAGGCAGCACAG NGG 10 8 17 8 24 0 100 5 10
    240613134013_k571 CTTGTCTGTGCTGCCTCCCGGCTTGTGAGCCG(+AGGACA)GATCCGCCTTACATTATTAAAGTTGTCAGCA NGG 10 8 17 8 24 0 100 5 10
    240613134012_9jyt GCCAAGTGCTGCTCTGCAGTCCGTGAAGCACG(+AGGACA)GTACCCGCATCCGGACCATCATGACTATAGG NGG 10 8 17 8 24 0 100 5 10
    240613134010_1a5l CACTCCCTATAGTCATGATGGTCCGGATGCGG(+AGGACA)GTACCGTGCTTCACGGACTGCAGAGCAGCAC NGG 10 8 17 8 24 0 100 5 10
    240613134008_731g GGTACAGCTGGATGCTGCAGAGGAGGGACTGG(+AGGACA)GTACCATGATGGCTCCCCTAGGGAGGCAGCA NGG 10 8 17 8 24 0 100 5 10
    240613134007_hpow TCTCCTGCTGCCTCCCTAGGGGAGCCATCATG(+AGGACA)GTACCCAGTCCCTCCTCTGCAGCATCCAGCT NGG 10 8 17 8 24 0 100 5 10
    240613134005_u922 GGTACAGCTGGATGCTGCAGAGGAGGGACTGG(+AGGACA)GTACCATGATGGCTCCCCTAGGGAGGCAGCA NGG 10 8 17 8 24 0 100 5 10
    240613134004_sglc TCTCCTGCTGCCTCCCTAGGGGAGCCATCATG(+AGGACA)GTACCCAGTCCCTCCTCTGCAGCATCCAGCT NGG 10 8 17 8 24 0 100 5 10
    240613134003_nzys GTGTGTGGTTTCCATCCCAAGGATGCCTGGTG(+AGGACA)GTACCACGGCTCCCAGGAAGAAGGTTGGAGA NGG 10 8 17 8 24 0 100 5 10
    240613134001_tspf CACACTCTCCAACCTTCTTCCTGGGAGCCGTG(+AGGACA)GTACCACCAGGCATCCTTGGGATGGAAACCA NGG 10 8 17 8 24 0 100 5 10