Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613133823_hxkx ACGTGGGGCCCATTTTGTTGGGGGCAGCCTGG(+AGGACA)GATCCCTCCATTTCTTGACATGTCCTAGCTT NGG 10 8 17 8 24 0 100 5 10
    240613133821_wtxy CCTTTCTCCACAAGCAAGGCATGGCGTACCAG(+AGGACA)GATCCCCACACTGTCCTGGCCTACTTTCTAC NGG 10 8 17 8 24 0 100 5 10
    240613133819_di18 TGAAGGTAGAAAGTAGGCCAGGACAGTGTGGG(+AGGACA)GATCCTGGTACGCCATGCCTTGCTTGTGGAG NGG 10 8 17 8 24 0 100 5 10
    240613133818_jxzx GGACTCGAGTGGCTCACAACCATCTGTAATAG(+AGGACA)GATCCGATGCCCTCTTCTGGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240613133817_pp4b GCTGTCTTCAGACACACCAGAAGAGGGCATCG(+AGGACA)GATCCTATTACAGATGGTTGTGAGCCACTCG NGG 10 8 17 8 24 0 100 5 10
    240613133814_i1f1 ATACCAGGACCCACGTGTGCTAGTGTCCCTGG(+AGGACA)GATCCCAGTTCACAGAAGAGCACCAAGGCCT NGG 10 8 17 8 24 0 100 5 10
    240613133812_bfey CTTCCAGGCCTTGGTGCTCTTCTGTGAACTGG(+AGGACA)GATCCCAGGGACACTAGCACACGTGGGTCCT NGG 10 8 17 8 24 0 100 5 10
    240613133809_rnsb CCTTAAGCTTATCTGCCTATTGATATCCCAGG(+AGGACA)GATCCTGAAACAGGCCCCTGCGCATACAGAG NGG 10 8 17 8 24 0 100 5 10
    240613133808_d0pq TGTTCCTCTGTATGCGCAGGGGCCTGTTTCAG(+AGGACA)GATCCCTGGGATATCAATAGGCAGATAAGCT NGG 10 8 17 8 24 0 100 5 10
    240613133807_p75u GGATTGCCAGCCTCCCTCAGCAGGGGCACTTG(+AGGACA)GTACCCCCATAAGGGTGGCATGCCCCAGTCC NGG 10 8 17 8 24 0 100 5 10