Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613133407_vyhi TAAATCGTTAATCTGATCCTGATTTAACTTTG(+AGGACA)GTACCTAGTATCTGTCTAGAAAAGTGTCCAC NGG 10 8 17 8 24 0 100 5 10
    240613133405_jd6k TGCTCCTAGTCACCCATTGGATGCAGCACAGG(+AGGACA)GTACCCAATGAAGGAGCTAAAGAAAGTACCT NGG 10 8 17 8 24 0 100 5 10
    240613133404_g76k TCTTTAGGTACTTTCTTTAGCTCCTTCATTGG(+AGGACA)GTACCCTGTGCTGCATCCAATGGGTGACTAG NGG 10 8 17 8 24 0 100 5 10
    240613133402_jab3 CATAGTGAGGAAATGAAATGATGCACTTAATG(+AGGACA)GTACCATCTCACTGTCAAGATAAAATACCCA NGG 10 8 17 8 24 0 100 5 10
    240613133401_rh0v GCAGCTGGGTATTTTATCTTGACAGTGAGATG(+AGGACA)GTACCATTAAGTGCATCATTTCATTTCCTCA NGG 10 8 17 8 24 0 100 5 10
    240613133400_q6y8 ATTGTTGAAAGTCAAACTTATTGTGAGTTAAG(+AGGACA)GATCCATAAAATTCTTCTGTTGCAGTTAGGG NGG 10 8 17 8 24 0 100 5 10
    240613133359_ntma AGTATCCCTAACTGCAACAGAAGAATTTTATG(+AGGACA)GATCCTTAACTCACAATAAGTTTGACTTTCA NGG 10 8 17 8 24 0 100 5 10
    240613133358_8n6l AATCCACTCAGGACCACACAGTAACACATGTG(+AGGACA)GATCCTGGCACCCCCACTTCTGCCTCCAACT NGG 10 8 17 8 24 0 100 5 10
    240613133356_c3nt CTGGAAGTTGGAGGCAGAAGTGGGGGTGCCAG(+AGGACA)GATCCACATGTGTTACTGTGTGGTCCTGAGT NGG 10 8 17 8 24 0 100 5 10
    240613133355_qmoe CTCTACAAAGATGTGATGCTGGAGATCTTCAG(+AGGACA)GATCCTCACTACTATAGGCTACACCTGGGAA NGG 10 8 17 8 24 0 100 5 10