Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613133354_k7ej ATGTCTTCCCAGGTGTAGCCTATAGTAGTGAG(+AGGACA)GATCCTGAAGATCTCCAGCATCACATCTTTG NGG 10 8 17 8 24 0 100 5 10
    240613133352_qcr7 AATAGCCACATGGGTACTGAGAATTAATCCTG(+AGGACA)GATCCTCTGAAAATGCCAGGTAATCAATGAG NGG 10 8 17 8 24 0 100 5 10
    240613133351_6qdt GACGGCTCATTGATTACCTGGCATTTTCAGAG(+AGGACA)GATCCAGGATTAATTCTCAGTACCCATGTGG NGG 10 8 17 8 24 0 100 5 10
    240613133349_2o61 ATGGCATTGTGCAGGCAGATTTGGTGCTGGAG(+AGGACA)GATCCAAAGGCAGCCAGACAATCTCCTCTGA NGG 10 8 17 8 24 0 100 5 10
    240613133347_w89y CCAGTTCAGAGGAGATTGTCTGGCTGCCTTTG(+AGGACA)GATCCTCCAGCACCAAATCTGCCTGCACAAT NGG 10 8 17 8 24 0 100 5 10
    240613133346_ar50 TTCTTTGGGTTTGTCGTTGGATTTGGAGCGAG(+AGGACA)GATCCATTTTCTCTTTACTCTGTCATTTGTC NGG 10 8 17 8 24 0 100 5 10
    240613133345_10gh ACAGTGACAAATGACAGAGTAAAGAGAAAATG(+AGGACA)GATCCTCGCTCCAAATCCAACGACAAACCCA NGG 10 8 17 8 24 0 100 5 10
    240613133343_az6h TCACCTAGAGACCCTGTGTAGATTTAACCAAG(+AGGACA)GTACCAAGAAAGTCCTGATGTTACCACAAGG NGG 10 8 17 8 24 0 100 5 10
    240613133342_0cly GTGGTCCTTGTGGTAACATCAGGACTTTCTTG(+AGGACA)GTACCTTGGTTAAATCTACACAGGGTCTCTA NGG 10 8 17 8 24 0 100 5 10
    240613133341_azfu TGCAATAGCTACAGACCAAACTGGCACAAACG(+AGGACA)GATCCTGTCCAACTGCATTGGTTTTCAAGGT NGG 10 8 17 8 24 0 100 5 10