Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613132650_nv95 CCCACATGTTTTCCAATTCACTGGTAAATGTG(+AGGACA)GTACCTCCCAGGCTGCAGATTCTCCTGGTAT NGG 10 8 17 8 24 0 100 5 10
    240613132648_m4uv GACAGCAAAGCCATCACAAAGTGGGCTGCGGG(+AGGACA)GATCCAGCCACAGCCACCATGGGCTGAGCAC NGG 10 8 17 8 24 0 100 5 10
    240613132646_zzjl ACATGGTGCTCAGCCCATGGTGGCTGTGGCTG(+AGGACA)GATCCCCGCAGCCCACTTTGTGATGGCTTTG NGG 10 8 17 8 24 0 100 5 10
    240613132645_y2nn TGTTTCTGGGGCCTCCTTCACGGATTCATATG(+AGGACA)GTACCTTCCTTCTAAACTTGAGGAAGGCATT NGG 10 8 17 8 24 0 100 5 10
    240613132644_pssk AGACAAATGCCTTCCTCAAGTTTAGAAGGAAG(+AGGACA)GTACCATATGAATCCGTGAAGGAGGCCCCAG NGG 10 8 17 8 24 0 100 5 10
    240613132641_8vdp ACTTCTCCCTGTCAGCCCCAGCTGGCCCATGG(+AGGACA)GATCCTGGGACAGCCGACTCATGACCCACCT NGG 10 8 17 8 24 0 100 5 10
    240613132639_1f3m TGTCCAGGTGGGTCATGAGTCGGCTGTCCCAG(+AGGACA)GATCCCATGGGCCAGCTGGGGCTGACAGGGA NGG 10 8 17 8 24 0 100 5 10
    240613132637_7spt AGGGCAGCATCTCCTGGGAAAACGAACTAGAG(+AGGACA)GATCCAGAGCCTTGTGGCCCTTTCACGGTCA NGG 10 8 17 8 24 0 100 5 10
    240613132636_w430 TTCTCTGACCGTGAAAGGGCCACAAGGCTCTG(+AGGACA)GATCCTCTAGTTCGTTTTCCCAGGAGATGCT NGG 10 8 17 8 24 0 100 5 10
    240613132635_riii GGCTCCCCTGCCTCAGTCTGCAAGGCTACTTG(+AGGACA)GTACCTCCTCACGCTCCGAGAAGTCTCACAC NGG 10 8 17 8 24 0 100 5 10