Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613132618_kvyg AACCCCAGGCAGTGGGAAACATTCCTTTAGTG(+AGGACA)GTACCGATGGAGTACCTGTCGATATCTGTGG NGG 10 8 17 8 24 0 100 5 10
    240613132617_crz3 CCAGCAGAGAACAAGCAGCCATGAGTCGGCTG(+AGGACA)GATCCTGTGCTAATGTGACGCTCCAAACTTA NGG 10 8 17 8 24 0 100 5 10
    240613132616_lj52 CTCAGTAAGTTTGGAGCGTCACATTAGCACAG(+AGGACA)GATCCAGCCGACTCATGGCTGCTTGTTCTCT NGG 10 8 17 8 24 0 100 5 10
    240613132614_rq62 CTTGGAGTCCCTAGATTAATGCTGGAGATGGG(+AGGACA)GATCCCAAGGTCTCCCAATGCCACTGGAGTC NGG 10 8 17 8 24 0 100 5 10
    240613132611_quq1 TCTAGGACTCCAGTGGCATTGGGAGACCTTGG(+AGGACA)GATCCCCATCTCCAGCATTAATCTAGGGACT NGG 10 8 17 8 24 0 100 5 10
    240613132610_rixr TGGGCTCTATGAATGATGCAACACATTAGATG(+AGGACA)GTACCTCCAAGGCTGGCTTCTACCATGAGTT NGG 10 8 17 8 24 0 100 5 10
    240613132608_rwdv AGGGTAACTCATGGTAGAAGCCAGCCTTGGAG(+AGGACA)GTACCATCTAATGTGTTGCATCATTCATAGA NGG 10 8 17 8 24 0 100 5 10
    240613132607_tsqj GAGGCTCCAGCCAAGATTGACAGATCCAGGTG(+AGGACA)GATCCTGATCCATTATTTAGCTATCTCTTCC NGG 10 8 17 8 24 0 100 5 10
    240613132606_7zxc TCACTGGAAGAGATAGCTAAATAATGGATCAG(+AGGACA)GATCCACCTGGATCTGTCAATCTTGGCTGGA NGG 10 8 17 8 24 0 100 5 10
    240613132604_3jds AAAGGGGCAGTGATGCTGAGCTGATCTAGGAG(+AGGACA)GATCCCTGGACCGTGCTCCATAGCATCAAAC NGG 10 8 17 8 24 0 100 5 10