Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613132532_27dy TTGTGGCCCAGATCTGAGTCCCAGCCTTGAGG(+AGGACA)GATCCTAAGAGCTGCTTGCTACTGGAGTCTG NGG 10 8 17 8 24 0 100 5 10
    240613132530_pnbt AGCACATGGTGGCTCAGAACCATCTGTAATGG(+AGGACA)GATCCGATGCCCTGTTCTGGTGTCTGAAGAT NGG 10 8 17 8 24 0 100 5 10
    240613132529_9pgz TAGCTATCTTCAGACACCAGAACAGGGCATCG(+AGGACA)GATCCCATTACAGATGGTTCTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240613132527_n16a TACCCCAAAAAGGTGAGGTGTCCACAGCTGGG(+AGGACA)GTACCACCAGTATACAGACCTTTAGAATTTG NGG 10 8 17 8 24 0 100 5 10
    240613132526_0owb ATGGACAAATTCTAAAGGTCTGTATACTGGTG(+AGGACA)GTACCCCAGCTGTGGACACCTCACCTTTTTG NGG 10 8 17 8 24 0 100 5 10
    240613132524_odsg ACCAACTTTTAGGTTGTGATACTTTCCTCCTG(+AGGACA)GTACCTGGGGGGAGGGGAGTATGCAGTTGGT NGG 10 8 17 8 24 0 100 5 10
    240613132521_scpz AAAGCACCAACTGCATACTCCCCTCCCCCCAG(+AGGACA)GTACCAGGAGGAAAGTATCACAACCTAAAAG NGG 10 8 17 8 24 0 100 5 10
    240613132520_lizg TTTCTTAGCTCTAGACCCTTCTGGTCTCTTTG(+AGGACA)GTACCTAGAACAATGTTTTATATTTACAGCT NGG 10 8 17 8 24 0 100 5 10
    240613132518_bjt9 TAAACAGCTGTAAATATAAAACATTGTTCTAG(+AGGACA)GTACCAAAGAGACCAGAAGGGTCTAGAGCTA NGG 10 8 17 8 24 0 100 5 10
    240613132517_8ze3 CAGGGTGAGGTGCCTGCTGGGCACAGACTGTG(+AGGACA)GATCCACTGTCTTCCTAGAGCAGGCCACATG NGG 10 8 17 8 24 0 100 5 10