Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613130950_t2h4 TCCAGCTTCTGCCAAGCTCCCCTAAGGAATTG(+AGGACA)GATCCAAAGTAAAATGGCCCATAGCTCTCCT NGG 10 8 17 8 24 0 100 5 10
    240613130949_efrv TGACCAGGAGAGCTATGGGCCATTTTACTTTG(+AGGACA)GATCCAATTCCTTAGGGGAGCTTGGCAGAAG NGG 10 8 17 8 24 0 100 5 10
    240613130946_je6d GAGGAGGTGTAGACTGGGAACTAGTGCCTGGG(+AGGACA)GTACCCCATCTTGAGGTCAGCAGCAAGTGGG NGG 10 8 17 8 24 0 100 5 10
    240613130944_arzk GTGGCCCCACTTGCTGCTGACCTCAAGATGGG(+AGGACA)GTACCCCAGGCACTAGTTCCCAGTCTACACC NGG 10 8 17 8 24 0 100 5 10
    240613130942_bxsd GCAAGCAAAAGGGAGGACTCCCAGAGCTTTGG(+AGGACA)GATCCAGTTGTGCCTCCCGGCTGACTGGCCT NGG 10 8 17 8 24 0 100 5 10
    240613130941_8k1q AGCGAAGGCCAGTCAGCCGGGAGGCACAACTG(+AGGACA)GATCCCAAAGCTCTGGGAGTCCTCCCTTTTG NGG 10 8 17 8 24 0 100 5 10
    240613130938_las6 AGACCTGACTCAGATGCCACTCTGACCCCAAG(+AGGACA)GTACCCTGCCCTTCGTGTGTTTTAACAAGCC NGG 10 8 17 8 24 0 100 5 10
    240613130937_exeh TTATTGGCTTGTTAAAACACACGAAGGGCAGG(+AGGACA)GTACCTTGGGGTCAGAGTGGCATCTGAGTCA NGG 10 8 17 8 24 0 100 5 10
    240613130935_qpsw TCCTCTGAGATCCCCACAAGTTCCTTTCTCGG(+AGGACA)GATCCTTTCACCCCTACCTCTTCCTGCCTGT NGG 10 8 17 8 24 0 100 5 10
    240613130934_nl8l CTCAAACAGGCAGGAAGAGGTAGGGGTGAAAG(+AGGACA)GATCCCGAGAAAGGAACTTGTGGGGATCTCA NGG 10 8 17 8 24 0 100 5 10