Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240708102302_3c9d aaaacgctttggattccccgggcctgggtggggaga(-gagagctgggtgccccct)gtatcccccacctccagcgcctcatgagccgatcctcggcccc NGG 10 8 17 8 24 0 100 5 10
    240708101809_qkc1 aaaacgctttggattccccgggcctgggtggggagagagag(-ctg)ggtgccccctgtatcccccacctccagcgcctcatgagccgatcctcggcccc NGG 10 8 17 8 24 0 100 5 10
    240708100604_m67f CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240707125206_ten3 GAGAGGGCAAGGAATGGGGGTGGGGGCTGGGGTGTGGATGCACAGTGAGGGAGACACTTCTCCAGATGGAAGAGTCACGCGTGGGTTCGTTCAAATGCGGGTGAGCGGGGCCTGAGGACTGGGAAAGGGACCCGAGGGAAGGAGGGGAGCGTGCAGCCCTGCCCCGGCCCAGCCCTGCCCTGGCCCAGCCCTGCCCCCTGCCCCTCAGGCGTGAGCCCCTCGCTCCAGTGCC(+AGTGTGCCTCCAGTGCCTCGCTCCA)GTGTGCGGAGACAGCAGCAGCGGGAAGCACTATGGCATCTATGCCTGCAACGGCTGCAGCGGCTTCTTCAAGAGGAGCGTACGGCGGAGGCTCATCTACAGGTGAGTGCGGTGGGCC NGG 10 8 17 8 60 0 100 5 10
    240707005616_ghoj CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240705112554_it6e GTTATTTGGGCCAAAGCGAGTCACAGAGGGTGGAAGATTCCATTCCTTTA(/C)TGAAAAGAGCTATAAATTCACATTTTAAAGGAAGGGTGGGGAAATGGAGCCATTTTTGCAATTCACCCAGCGTAGTCACTGAAGCCAAACATTTTTTCCATCTGTTTGGTTTGTGGATTTTTTGTTAGTGATGT NGG 10 8 17 8 24 0 100 5 10
    240705112525_yi16 GTTATTTGGGCCAAAGCGAGTCACAGAGGGTGGAAGATTCCATTCCTTTA(/C)TGAAAAGAGCTATAAATTCACATTTTAAAGGAAGGGTGGGGAAATGGAGCCATTTTTGCAATTCACCCAGCGTAGTCACTGAAGCCAAACATTTTTTCCATCTGTTTGGTTTGTGGATTTTTTGTTAGTGATGT NGG 10 8 17 8 24 0 100 5 10
    240705112431_357q GTTATTTGGGCCAAAGCGAGTCACAGAGGGTGGAAGATTCCATTCCTTTA(/C)TGAAAAGAGCTATAAATTCACATTTTAAAGGAAGGGTGGGGAAATGGAGCCATTTTTGCAATTCACCCAGCGTAGTCACTGAAGCCAAACATTTTTTCCATCTGTTTGGTTTGTGGATTTTTTGTTAGTGATGT NGG 10 8 17 8 24 0 100 5 10
    240705084708_4dbp GAGGGAGGAGAGAGGGCAAGGAATGGGGGTGGGGGCTGGGGTGTGGATGCACAGTGAGGGAGACACTTCTCCAGATGGAAGAGTCACGCGTGGGTTCGTTCAAATGCGGGTGAGCGGGGCCTGAGGACTGGGAAAGGGACCCGAGGGAAGGAGGGGAGCGTGCAGCCCTGCCCCGGCCCAGCCCTGCCCTGGCCCAGCCCTGCCCCCTGCCCCTCAGGCGTGAGCCCCTCGCTCCAGTGCCGCGTGTGCGGAGACAGCAG(-CAGCGGGA)AGCACTATGGCATCTATGCCTGCAACGGCTGCAGCGGCTTCTTCAAGAGGAGCGTACGGCGGAGGCTCATCTACAGGTGAGTGCGGTGGGCCCTGCTGGGCGTCTGCCCCTGAGGGGTTCTGGAGGGGTGAGGGGGTGCTCAGGGGAAGAGG NGG 10 8 17 8 24 0 100 5 10
    240704214238_2s11 ACCAGGGCCAGGCAGCTGAGGGCAGCGAGCCGAGAGATGCCAGGACAGGAGAGGAGAGGATCTGGTACCACTTAAGACTCACCTGTGATGACTCCAGGTGCCTGGGCAGCCATGACAGCCTGGGGCAAAGTGCCCAGGGGCTGGGCCAGGGTC(+GGGGCTGGGCCAGGGTCAA)TGCTGGGGACACCAGTCCAGGTGTGCCCAGGGTACCCAGCACCGCTGCTCCGGCCACTGCTTCCTGCAACCCAAAAGGTCACCGTGCTCAGTCCCTGGTCTGGCTACTCAAGACCACCTTGAATCAGTCTCCAAGGAATCAGGGG NGG 10 8 17 8 24 0 20 5 10