Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".
  • Substitution is indicated by backslash "/", and the example is used to replace A by T.
  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position
  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.
  • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240716084519_m4lq GCACGAGTCATTTACTCTTTTGTTTCTATTTCCAAACGAGTCATTTACTCTTTTGTTTCTATTTCCAAACAGGTCTGATGGGAAAATCTCAGAACAGTCAGACGCAAAGCTCAAAGAGATTGTAACAAACTTCTTGGC(/G)TGGGTTTGAACCATAAAGCCCTGTCACTGTCACCAGATACTGCTTTGGTTTTGTCATTTATTCTGGTAAAATCAGCACCATTTGTAAAGGGTTACTCTTGTATTCCTGATGTACAGAAATCACATGAATAAA NGG 10 8 17 8 24 0 100 5 10
    240716084345_2fx9 GCACGAGTCATTTACTCTTTTGTTTCTATTTCCAAACGAGTCATTTACTCTTTTGTTTCTATTTCCAAACAGGTCTGATGGGAAAATCTCAGAACAGTCAGACGCAAAGCTCAAAGAGATTGTAACAAACTTCTTGG(/T)CTGGGTTTGAACCATAAAGCCCTGTCACTGTCACCAGATACTGCTTTGGTTTTGTCATTTATTCTGGTAAAATCAGCACCATTTGTAAAGGGTTACTCTTGTATTCCTGATGTACAGAAATCACATGAATAAA NGG 10 8 17 8 24 0 100 5 10
    240715222724_zlhn CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240715164647_wxqp CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240714001314_q143 gccggactgcccccggctgcctcctgcccggagaagtgcgccctgtttaacagcgtctcctccagcctctgcaaacagtgcacggagaagcct(+TT)taaacacacccccgaaaaacacaagaccgacccaaaatctagaggaaagcgaaaagctgctccccac NGG 10 8 17 8 24 0 100 5 10
    240713111228_fqkk ATCTGCGCACGCGCGGCCTCACCCCGCCCCTCGCGTCCCCGGCGGAGCGA(/T)TGGGACGGGGGCGGGCAGCCGGGGTGAGGAGGCCGCCGGGAGTCCCTAGATTGCCGGCGCGCTCCGCCTCCGCCGCCGCTCCACCGCCGCCGCTTCCCTCGCCGCGTCACGGCTCCCGGGACCGCCCTCCTCTGGCCCCTCCTACTCGCTCCCTTTCCCCCTCCTCTTACCCTCCCCCTCCGACCGATCGGTGACTTAAG NGG 10 8 17 8 24 0 100 5 10
    240713110821_d63e ATCTGCGCACGCGCGGCCTCACCCCGCCCCTCGCGTCCCCGGCGGAGCGA(/T)TGGGACGGGGGCGGGCAGCCGGGGTGAGGAGGCCGCCGGGAGTCCCTAGATTGCCGGCGCGCTCCGCCTCCGCCGCCGCTCCACCGCCGCCGCTTCCCTCGCCGCGTCACGGCTCCCGGGACCGCCCTCCTCTGGCCCCTCCTACTCGCTCCCTTTCCCCCTCCTCTTACCCTCCCCCTCCGACCGATCGGTGACTTAA NGG 10 8 17 8 24 0 100 5 10
    240713110337_5383 ATCTGCGCACGCGCGGCCTCACCCCGCCCCTCGCGTCCCCGGCGGAGCGA(/T)TGGGACGGGGGCGGGCAGCCGGGGTGAGGAGGCCGCCGGGAGTCCCTAGATTGCCGGCGCGCTCCGCCTCCGCCGCCGCTCCACCGCCGCCGCTTCCCTCGCCGCGTCACGGCTCCCGGGACCGCCCTCCTCTGGCCCCTCCTACTCGCTCCCTTTCCCCCTCCTCTTACCCTCCCCCTCCGACCGATCGGTGACTTAA NGG 10 8 17 8 24 0 100 5 10
    240712212059_tmp9 GTCCAGAATGTCTTCGTGGATACCCATGATCTTCGCTTCCCCTGGGTGGCCTTCTTTGCCAGCAAAAGAATCCGGGCTGGGACAGAACTTACTTGGGACTA(/T)CAACTACGAGGTGGGCAGTGTGGAAGGCAAGGAGCTACTCTGTTGCTGTGGGGCCATTGAATGCAGAGGACGTCTTCTTTAG NGG 10 8 17 8 24 0 100 5 10
    240712211834_8gwt CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10