Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240729095438_fl3l gcctcccacctgcgcaagctgcgtaagcggctcctccgcgatgccgatgacctgcagaagc(/t)gcctggcagtgtaccaggccggggcccgcgagggcgccgagcgcggcctcagcgccatccgcgagcgcc NGG 10 8 17 8 24 0 100 5 10
    240729020720_jh5m GTACACGGAGTGGATTTTGGAGGCCATCAAAAAAGTCAAGAAGCAGAAACAGCGAC(/A)CTTCAGAGGAAAGGATCTGCAATGCCGTGTCCTCATCCCATGGGCTGGAC NGG 10 8 17 8 24 0 100 5 10
    240728225725_p34w ACCTGAAGATGAGAGCGGCAGAGACTATAGGGAGAGGGAACGGGAATATGAACGAGATCAGGAGCG(/A)CATACTTCGAGAAAGAGAGAGGCTGAAGCGGCAAGAAGAAGAGCGCCGTAGGCAGAAGGAGCGCTATGAGAAAGAGAAGACTTTTAAGAGAAAAGAAGAAGAAATGAAAAAAGAGAAAGACACACTTCGGGATAAAGGAAAGAAGG NGG 10 8 17 8 24 0 100 5 10
    240728225621_5030 ACCTGAAGATGAGAGCGGCAGAGACTATAGGGAGAGGGAACGGGAATATGAACGAGATCAGGAGCG(/A)CATACTTCGAGAAAGAGAGAGGCTGAAGCGGCAAGAAGAAGAGCGCCGTAGGCAGAAGGAGCGCTATGAGAAAGAGAAGACTTTTAAGAGAAAAGAAGAAGAAATGAAAAAAGAGAAAGACACACTTCGGGATAAAGGAAAGAAGG NGG 10 8 17 8 24 0 100 5 10
    240727053858_2mcr gtcctgggtcctcaggaactttcattatcaacagcacacaggtgaactccagaaagaagaagctatg(+TTT)gcagtgattctggagagcatctttctgaagcgatcccaacagaaaaagaaaacatcacctctaaacttcaagaagcgcctgtttctcttgaccgtgcacaaactctcctactatgagtatgactttgaacgtggggtaagtttctcgactatgaaaactgagtttcaagatatcaag NGG 10 8 17 8 24 0 100 5 10
    240727053720_kqqb gtcctgggtcctcaggaactttcattatcaacagcacacaggtgaactccagaaagaagaagctatg(+gcc)gcagtgattctggagagcatctttctgaagcgatcccaacagaaaaagaaaacatcacctctaaacttcaagaagcgcctgtttctcttgaccgtgcacaaactctcctactatgagtatgactttgaacgtggggtaagtttctcgactatgaaaactgagtttcaagatatcaag NGG 10 8 17 8 24 0 100 5 10
    240727053635_7xun gtcctgggtcctcaggaactttcattatcaacagcacacaggtgaactccagaaagaagaagctatg(-gcc)gcagtgattctggagagcatctttctgaagcgatcccaacagaaaaagaaaacatcacctctaaacttcaagaagcgcctgtttctcttgaccgtgcacaaactctcctactatgagtatgactttgaacgtggggtaagtttctcgactatgaaaactgagtttcaagatatcaag NGG 10 8 17 8 24 0 100 5 10
    240727053210_aigi CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240726210836_da92 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240726145830_0g42 atggaaagaagtgactggagtccatagtatggaagacaacggcatcaagcatggagggctagacctcacgactaacaattcctcctcgactacctcctccac(/a)cacgtccaaagcatcaccacccatcacacatcattccatagtgaacggacagtcttcagttctgaatgcaaggcgggacaggtgagcctcctgcgctgcgc NGG 10 8 17 8 24 0 100 5 10