Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240726145829_e4hq atggaaagaagtgactggagtccatagtatggaagacaacggcatcaagcatggagggctagacctcacgactaacaattcctcctcgactacctcctccac(/a)cacgtccaaagcatcaccacccatcacacatcattccatagtgaacggacagtcttcagttctgaatgcaaggcgggacaggtgagcctcctgcgctgcgc NGG 10 8 17 8 24 0 100 5 10
    240726105334_vc5y atggaaagaagtgactggagtccatagtatggaagacaacggcatcaagcatggagggctagacctcacgactaacaattcctcctcgactacctcctccac(/a)cacgtccaaagcatcaccacccatcacacatcattccatagtgaacggacagtcttcagttctgaatgcaaggcgggacaggtgagcctcctgcgctgcgc NGG 10 8 17 8 24 0 100 5 10
    240725212903_m52k ATCTGCGCACGCGCGGCCTCACCCCGCCCCTCGCGTCCCCGGCGGAGCGAT(/A)GGGACGGGGGCGGGCAGCCGGGGTGAGGAGGCCGCCGGGAGTCCCTAGATTGCCGGCGCGCTCCGCCTCCGCCGCCGCTCCACCGCCGCCGCTTCCCTCGCCGCGTCACGGCTCCCGGGACCGCCCTCCTCTGGCCCCTCCTACTCGCTCCCTTTCCCCCTCCTCTTACCCTCCCCCTCCGACCGATCGGTGACTTAAGCAACGGAGCGCGGTGAAGCCCATTTTTCTCCTTCCTCGCAGCCGCGCCGGGCAGTTCGCGGCGCGCGGCCCCCACGCTCCCGCCCGAGATGAAT NGG 10 8 17 8 24 0 100 5 10
    240724182754_pjeh TTTTATCTTTCTCTCGACCACTTAAAACTTCAGACTTCCTGTCCTGCTGGTATCATGGAGAAAGTCCAATACCTCACTCGCTCAGCTATAAGA(+AGA)GCCTCAACCATTGAAATGCCTCAACAAGCACGTCAAAAGCTACAGAATCTATTTATCAATTTCTGTCTCATCTTAATATGTCTCTTGCTGATCTGTATCATCGTGATGCTTCTC NGG 10 8 17 8 24 0 100 5 10
    240724182701_eze3 TTTTATCTTTCTCTCGACCACTTAAAACTTCAGACTTCCTGTCCTGCTGGTATCATGGAGAAAGTCCAATACCTCACTCGCTCAGCTATAAGA(+AGA)GCCTCAACCATTGAAATGCCTCAACAAGCACGTCAAAAGCTACAGAATCTATTTATCAATTTCTGTCTCATCTTAATATGTCTCTTGCTGATCTGTATCATCGTGATGCTTCTC NGG 10 8 17 8 24 0 100 5 10
    240724103822_st1a CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240724054030_36ui CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240723073949_x8om CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCG(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240723073706_uggv CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240723073550_54oe CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10