Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613120749_efpo GGACTCTGCTCAGAAGGCAGAGCCTACAGAAG(+AGGACA)GTACCAAATTTACTGTTGAGTGATTGAGATT NGG 10 8 17 8 24 0 100 5 10
    240613120747_p51t AAAATCTGAATATTTTTTTAAGATCACCTAAG(+AGGACA)GTACCATTTTAAAGATTCCAAGACCCTAGAA NGG 10 8 17 8 24 0 100 5 10
    240613120746_hkb6 GAATTTTCTAGGGTCTTGGAATCTTTAAAATG(+AGGACA)GTACCTTAGGTGATCTTAAAAAAATATTCAG NGG 10 8 17 8 24 0 100 5 10
    240613120745_oji2 TGCCAGATGGGGAAAACAAGAAGGACAAATAG(+AGGACA)GATCCTGCATTGTTTTTGCTTCCTCTCCTGT NGG 10 8 17 8 24 0 100 5 10
    240613120744_vwhi AATTGACAGGAGAGGAAGCAAAAACAATGCAG(+AGGACA)GATCCTATTTGTCCTTCTTGTTTTCCCCATC NGG 10 8 17 8 24 0 100 5 10
    240613120742_9sgs CTACTCATAACTATCCTTATGACCTGAGTTGG(+AGGACA)GTACCACATAGTTTTGTCACTGAAAAATGGA NGG 10 8 17 8 24 0 100 5 10
    240613120741_ba19 TGTAATCCATTTTTCAGTGACAAAACTATGTG(+AGGACA)GTACCCAACTCAGGTCATAAGGATAGTTATG NGG 10 8 17 8 24 0 100 5 10
    240613120739_7lb8 AAATGAAGCTGATAAAACAAAGCTAAGACTGG(+AGGACA)GATCCTTGCAAGTATCTGATAAAACTAGCCT NGG 10 8 17 8 24 0 100 5 10
    240613120738_ip7o GAGTAAGGCTAGTTTTATCAGATACTTGCAAG(+AGGACA)GATCCCAGTCTTAGCTTTGTTTTATCAGCTT NGG 10 8 17 8 24 0 100 5 10
    240613120736_p35b CTGTGCCCCACAGAGGCCATAAGAGGGCCTTG(+AGGACA)GATCCCTATAACTAATTAGACTGTTTTAAGT NGG 10 8 17 8 24 0 100 5 10