Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613120834_uzbo TCGGGAACATTTGGGTCATCCCAGAGTGGGAG(+AGGACA)GATCCAGCTCCTAAACTGTTTAGGAGTTCCT NGG 10 8 17 8 24 0 100 5 10
    240613120833_ck8u CATCAGGACAAGATTTGTATAGGAATGCTGGG(+AGGACA)GATCCAAACTCAAGTGTACTTATACCAACCA NGG 10 8 17 8 24 0 100 5 10
    240613120831_y15b GGACTTGGTTGGTATAAGTACACTTGAGTTTG(+AGGACA)GATCCCCAGCATTCCTATACAAATCTTGTCC NGG 10 8 17 8 24 0 100 5 10
    240613120830_qivq CAAACAGTTTAATAGCAAATAAACAAAGGAAG(+AGGACA)GTACCACACTTGGCAGATACAAAATGACTTT NGG 10 8 17 8 24 0 100 5 10
    240613120829_d43x GACAAAAAGTCATTTTGTATCTGCCAAGTGTG(+AGGACA)GTACCTTCCTTTGTTTATTTGCTATTAAACT NGG 10 8 17 8 24 0 100 5 10
    240613120827_4mi3 TGTACTCCCTGAGCAGAGCAGCCAGCCTCCTG(+AGGACA)GATCCCCCACTGAGACCTGGAGGCCACCACA NGG 10 8 17 8 24 0 100 5 10
    240613120825_rc4q CTCTTTGTGGTGGCCTCCAGGTCTCAGTGGGG(+AGGACA)GATCCAGGAGGCTGGCTGCTCTGCTCAGGGA NGG 10 8 17 8 24 0 100 5 10
    240613120823_u1n4 TCCCTGCATGTATGCCTGCAGGTCAGAAGAGG(+AGGACA)GTACCAGATCCCGTTACAGATGGTTGTGAGC NGG 10 8 17 8 24 0 100 5 10
    240613120822_5aqx TGGTGGCTCACAACCATCTGTAACGGGATCTG(+AGGACA)GTACCCTCTTCTGACCTGCAGGCATACATGC NGG 10 8 17 8 24 0 100 5 10
    240613120820_zfcn AGTAGGCTAGTCTGGCTGGCCAGCACCACAGG(+AGGACA)GATCCTGTCTCTACCCTCCAGCATGGGCATC NGG 10 8 17 8 24 0 100 5 10