Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613120921_atyw GGGGGCACACTGTAACCCTGTGTTTCAGCAAG(+AGGACA)GTACCCATTTGGCACAATGGCCACAAGCCTC NGG 10 8 17 8 24 0 100 5 10
    240613120919_426v AGCTCAATGTGAGCAGGGGCTGCCGACTGAGG(+AGGACA)GTACCTGGCACATATCTTTTTCCATAACACA NGG 10 8 17 8 24 0 100 5 10
    240613120918_0a8a TCATCTGTGTTATGGAAAAAGATATGTGCCAG(+AGGACA)GTACCCTCAGTCGGCAGCCCCTGCTCACATT NGG 10 8 17 8 24 0 100 5 10
    240613120916_t9uu AGTGACATATAACGCATGCCACATTAATGTGG(+AGGACA)GATCCATCCACAGTGACTTAGGAAAGGAAGA NGG 10 8 17 8 24 0 100 5 10
    240613120914_ep1m GTTAGTCTTCCTTTCCTAAGTCACTGTGGATG(+AGGACA)GATCCCACATTAATGTGGCATGCGTTATATG NGG 10 8 17 8 24 0 100 5 10
    240613120912_e324 GGTTCCTGGTTCCTTCTGCTGATCCACACTGG(+AGGACA)GATCCAGCCCTCCTTTGCCTCAGCTGGTTTG NGG 10 8 17 8 24 0 100 5 10
    240613120911_losi AGCCCCAAACCAGCTGAGGCAAAGGAGGGCTG(+AGGACA)GATCCCAGTGTGGATCAGCAGAAGGAACCAG NGG 10 8 17 8 24 0 100 5 10
    240613120909_ftcv AAGCACCTCTTGTCTGCTGTAGTTTCAGTCAG(+AGGACA)GATCCAGCTCATTTTCCATGGACCTAACTGG NGG 10 8 17 8 24 0 100 5 10
    240613120908_32mk CTTGCCCAGTTAGGTCCATGGAAAATGAGCTG(+AGGACA)GATCCTGACTGAAACTACAGCAGACAAGAGG NGG 10 8 17 8 24 0 100 5 10
    240613120907_ix5m CCTGATCCTTTGGGCTCTCTGGAGGGTTCTGG(+AGGACA)GTACCTAACCCTCCTCTCTGATCCACCCACC NGG 10 8 17 8 24 0 100 5 10