Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613120905_3poj CCCAGGGTGGGTGGATCAGAGAGGAGGGTTAG(+AGGACA)GTACCCAGAACCCTCCAGAGAGCCCAAAGGA NGG 10 8 17 8 24 0 100 5 10
    240613120903_jri1 GGCTACTGCAAGTCTGGAATTTTCTCATTGGG(+AGGACA)GATCCATGAGGGTGCTTGCTTGTGTCATTCG NGG 10 8 17 8 24 0 100 5 10
    240613120902_np8b AGTGGCGAATGACACAAGCAAGCACCCTCATG(+AGGACA)GATCCCCAATGAGAAAATTCCAGACTTGCAG NGG 10 8 17 8 24 0 100 5 10
    240613120900_ktyi AGGTCCTGCTTCTGCTCAGCAGCTTGAGGGAG(+AGGACA)GTACCAGTGCCTCATTTTGTCTCTATCGGTT NGG 10 8 17 8 24 0 100 5 10
    240613120859_zs2s CACAGAACCGATAGAGACAAAATGAGGCACTG(+AGGACA)GTACCTCCCTCAAGCTGCTGAGCAGAAGCAG NGG 10 8 17 8 24 0 100 5 10
    240613120857_9dgi CTCTGCCAGCCTGCTCTGTTCCTCTGTCCCTG(+AGGACA)GATCCTCACTGGCAGCAGCTGTTACGCATTG NGG 10 8 17 8 24 0 100 5 10
    240613120855_ugbr TGCTCCAATGCGTAACAGCTGCTGCCAGTGAG(+AGGACA)GATCCAGGGACAGAGGAACAGAGCAGGCTGG NGG 10 8 17 8 24 0 100 5 10
    240613120854_ujjt CTAGCCACACCTGTAGGGCAAGGTCACTGCAG(+AGGACA)GTACCTCCCTGTCATTAGGCAGTGGACTTCG NGG 10 8 17 8 24 0 100 5 10
    240613120852_o9z1 CTTCCCGAAGTCCACTGCCTAATGACAGGGAG(+AGGACA)GTACCTGCAGTGACCTTGCCCTACAGGTGTG NGG 10 8 17 8 24 0 100 5 10
    240613120850_ordm ACCACATGGTGGCTCACAACCATCTGTAATGG(+AGGACA)GATCCAATATCCTCTTTTGGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10