Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613121012_o214 TGGGTATCTCCCAGCACACTTACTACTTCCTG(+AGGACA)GATCCTGAAAAGCATGGTTGTAAGAGACTGT NGG 10 8 17 8 24 0 100 5 10
    240613121009_o0kk TGTAGCAGTTTTAACCAAATTTCATTCCCCTG(+AGGACA)GTACCGTGGAGCTTTATTTTGCTATTGAACG NGG 10 8 17 8 24 0 100 5 10
    240613121007_kd35 CTTGGCGTTCAATAGCAAAATAAAGCTCCACG(+AGGACA)GTACCAGGGGAATGAAATTTGGTTAAAACTG NGG 10 8 17 8 24 0 100 5 10
    240613121006_f862 GCTAGGAATACAGTGATGGAAAAATAAACAAG(+AGGACA)GTACCTACTTCTTTGGAGACTGGAGTGGTTG NGG 10 8 17 8 24 0 100 5 10
    240613121004_7gg4 GGTTCCAACCACTCCAGTCTCCAAAGAAGTAG(+AGGACA)GTACCTTGTTTATTTTTCCATCACTGTATTC NGG 10 8 17 8 24 0 100 5 10
    240613121002_0ywr TTCCTGACTTCCAGCCTTGCCTGACTCACCGG(+AGGACA)GATCCTTATGTTTGGGCCTTCCCCATCCTAT NGG 10 8 17 8 24 0 100 5 10
    240613121001_svzf GTCCAATAGGATGGGGAAGGCCCAAACATAAG(+AGGACA)GATCCCGGTGAGTCAGGCAAGGCTGGAAGTC NGG 10 8 17 8 24 0 100 5 10
    240613120959_2av2 CTGAGATTACAGAAGTACCCAGAAGTGTCTGG(+AGGACA)GATCCCTGTCACATCTGTGTCATGTTGTTAT NGG 10 8 17 8 24 0 100 5 10
    240613120957_4gb9 TTCAAATAACAACATGACACAGATGTGACAGG(+AGGACA)GATCCCAGACACTTCTGGGTACTTCTGTAAT NGG 10 8 17 8 24 0 100 5 10
    240613120956_o4j9 TGATAGCCAACCTTGGTTTAGGCATCTCTCAG(+AGGACA)GTACCTACAATTTCCCAAGTTCCAATAAATT NGG 10 8 17 8 24 0 100 5 10