Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613121042_eb14 CCGTCCTGATTCTGCACAACCTCAGATCTCAG(+AGGACA)GTACCAGGCCACACAGGAGGCCTTTATGTCC NGG 10 8 17 8 24 0 100 5 10
    240613121040_c8ue ACCCGTTTGGCAGGGGCTCTAGTTGAGGGTTG(+AGGACA)GTACCCCAAGGAAGGATCATGATCGGCCGGA NGG 10 8 17 8 24 0 100 5 10
    240613121038_9sbq AGGAGTCCGGCCGATCATGATCCTTCCTTGGG(+AGGACA)GTACCAACCCTCAACTAGAGCCCCTGCCAAA NGG 10 8 17 8 24 0 100 5 10
    240613121037_5365 CTATGAAAACCTTCCCTTTGACAGTTCAGTTG(+AGGACA)GATCCAGCTCCTGCTAGCCACTGAATTAATT NGG 10 8 17 8 24 0 100 5 10
    240613121036_ur0i ACACAAATTAATTCAGTGGCTAGCAGGAGCTG(+AGGACA)GATCCAACTGAACTGTCAAAGGGAAGGTTTT NGG 10 8 17 8 24 0 100 5 10
    240613121035_9u70 TCTGTCTTCATAAAAGGGCAAGTTTGCTCACG(+AGGACA)GTACCCGAGAGCAGGCACTTAGACCTGAATA NGG 10 8 17 8 24 0 100 5 10
    240613121033_82gq CGCCATATTCAGGTCTAAGTGCCTGCTCTCGG(+AGGACA)GTACCGTGAGCAAACTTGCCCTTTTATGAAG NGG 10 8 17 8 24 0 100 5 10
    240613121032_b1o9 CCAGCTACTGCATCCAGTGGCCTGGGGCTCAG(+AGGACA)GATCCATTTTCTACATTCATAAGAATGCCCA NGG 10 8 17 8 24 0 100 5 10
    240613121031_7w6o GGGCATGGGCATTCTTATGAATGTAGAAAATG(+AGGACA)GATCCTGAGCCCCAGGCCACTGGATGCAGTA NGG 10 8 17 8 24 0 100 5 10
    240613121029_mx4s TATAGGACTTGATGAAATTAAGCAAGGTCCTG(+AGGACA)GATCCCAGAAACAGGTCCACAGGTTCTTCGG NGG 10 8 17 8 24 0 100 5 10