Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613121115_u6vr GTCCCCAAGTTCTCGAGTTGCTGCTTACTGAG(+AGGACA)GATCCTGTCACTTTTATAAGCAGTCAAAGCA NGG 10 8 17 8 24 0 100 5 10
    240613121113_0rji GATAACTTCACAACATTGGAGTGGTCAGAAAG(+AGGACA)GTACCTGTTAAATGTTACTAACGAAACAAAT NGG 10 8 17 8 24 0 100 5 10
    240613121112_407w AGAGCATTTGTTTCGTTAGTAACATTTAACAG(+AGGACA)GTACCTTTCTGACCACTCCAATGTTGTGAAG NGG 10 8 17 8 24 0 100 5 10
    240613121111_cccg TTCCTCTATAAATTTTAAGTCTTGTTGCAGAG(+AGGACA)GTACCCAGGAAGTCCTGCTGTCCTCTAATGG NGG 10 8 17 8 24 0 100 5 10
    240613121109_2hho ACAACCCATTAGAGGACAGCAGGACTTCCTGG(+AGGACA)GTACCTCTGCAACAAGACTTAAAATTTATAG NGG 10 8 17 8 24 0 100 5 10
    240613121107_rlxp ACCGTGGTTTCTGCTTCAGCCCCTGCATCTAG(+AGGACA)GATCCTGCTGTGTTTGAGATCTTGTCCTGAC NGG 10 8 17 8 24 0 100 5 10
    240613121106_kolb AGGAAGTCAGGACAAGATCTCAAACACAGCAG(+AGGACA)GATCCTAGATGCAGGGGCTGAAGCAGAAACC NGG 10 8 17 8 24 0 100 5 10
    240613121103_2yx2 TCCTCCAACCACCTACAACTCCATCTCCAGGG(+AGGACA)GATCCAATGCCTTCTTCTGGTCTTTGCAGGC NGG 10 8 17 8 24 0 100 5 10
    240613121102_ytie CAGGTGCCTGCAAAGACCAGAAGAAGGCATTG(+AGGACA)GATCCCCTGGAGATGGAGTTGTAGGTGGTTG NGG 10 8 17 8 24 0 100 5 10
    240613121100_b1m3 CTTAGCATTTTTACCTGCTGTTTGGCTCTGTG(+AGGACA)GTACCAAGTCATGGGAATTTCTCTCGGGGTC NGG 10 8 17 8 24 0 100 5 10