Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613121027_cgnw GCATACCGAAGAACCTGTGGACCTGTTTCTGG(+AGGACA)GATCCAGGACCTTGCTTAATTTCATCAAGTC NGG 10 8 17 8 24 0 100 5 10
    240613121026_li1t CGATGGCTCTATTAATACACACACGGTGACAG(+AGGACA)GTACCCTATTTAGACTAAGGCTACTAGAACA NGG 10 8 17 8 24 0 100 5 10
    240613121024_smn3 GAGTGTGTTCTAGTAGCCTTAGTCTAAATAGG(+AGGACA)GTACCTGTCACCGTGTGTGTATTAATAGAGC NGG 10 8 17 8 24 0 100 5 10
    240613121022_8vje GATACATCTACTCAGCATAGGAGAGCCCTGGG(+AGGACA)GTACCACTTACAGGCTATCTAGAATAGTTCC NGG 10 8 17 8 24 0 100 5 10
    240613121021_r75h GCTGTGGAACTATTCTAGATAGCCTGTAAGTG(+AGGACA)GTACCCCAGGGCTCTCCTATGCTGAGTAGAT NGG 10 8 17 8 24 0 100 5 10
    240613121019_ao28 CATGTGTGCTCGTTGTCTTAAGTCCCTGGAGG(+AGGACA)GTACCACACTGCTTTCATCCTGTCCCCTTGG NGG 10 8 17 8 24 0 100 5 10
    240613121018_6crh TTTTTCCAAGGGGACAGGATGAAAGCAGTGTG(+AGGACA)GTACCCTCCAGGGACTTAAGACAACGAGCAC NGG 10 8 17 8 24 0 100 5 10
    240613121016_wc1o ATAAAAAGAGTTCTGTGCTTGTGTGGAGATTG(+AGGACA)GATCCGAAGAAACTGGCTGCTGGGGTTTGGC NGG 10 8 17 8 24 0 100 5 10
    240613121015_eth0 CCCGGGCCAAACCCCAGCAGCCAGTTTCTTCG(+AGGACA)GATCCAATCTCCACACAAGCACAGAACTCTT NGG 10 8 17 8 24 0 100 5 10
    240613121014_5o1d AGATCACAGTCTCTTACAACCATGCTTTTCAG(+AGGACA)GATCCAGGAAGTAGTAAGTGTGCTGGGAGAT NGG 10 8 17 8 24 0 100 5 10