Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613120955_xac2 AACAGAATTTATTGGAACTTGGGAAATTGTAG(+AGGACA)GTACCTGAGAGATGCCTAAACCAAGGTTGGC NGG 10 8 17 8 24 0 100 5 10
    240613120953_rciu GACAAAATAGCCCATGGAAGCACAGTGAAGGG(+AGGACA)GTACCCTGACAGACAGATGAGCCACAGGCCC NGG 10 8 17 8 24 0 100 5 10
    240613120951_bafu TTGAAGGGCCTGTGGCTCATCTGTCTGTCAGG(+AGGACA)GTACCCCTTCACTGTGCTTCCATGGGCTATT NGG 10 8 17 8 24 0 100 5 10
    240613120949_sr4g CAGATGCCCTGGAACTGGAGTTACAGACTGGG(+AGGACA)GATCCTCTGGAAGAGCAGCCAAGGCTCTTAA NGG 10 8 17 8 24 0 100 5 10
    240613120947_xbco AGTGGTTAAGAGCCTTGGCTGCTCTTCCAGAG(+AGGACA)GATCCCCAGTCTGTAACTCCAGTTCCAGGGC NGG 10 8 17 8 24 0 100 5 10
    240613120945_ztqz TTCACACTAAGTAAGGGTTAAACCAGTCCCTG(+AGGACA)GATCCTTAGTGAACTATAGAGGAGGGAGTAC NGG 10 8 17 8 24 0 100 5 10
    240613120944_fv5b CTCAAGTACTCCCTCCTCTATAGTTCACTAAG(+AGGACA)GATCCAGGGACTGGTTTAACCCTTACTTAGT NGG 10 8 17 8 24 0 100 5 10
    240613120942_8num TTCCTCATGGTCTCCTCCTACATCAAGCCAGG(+AGGACA)GTACCCTCAATCCCCACTTTTGGCTCTTCTG NGG 10 8 17 8 24 0 100 5 10
    240613120940_7lw1 CTGGGCAGAAGAGCCAAAAGTGGGGATTGAGG(+AGGACA)GTACCCTGGCTTGATGTAGGAGGAGACCATG NGG 10 8 17 8 24 0 100 5 10
    240613120939_jhnx GTTGGTTTTGTTGTTTGTTTTTTCCGACGTTG(+AGGACA)GTACCTTGGAAAGGTTGTGTATTTTAAAGTC NGG 10 8 17 8 24 0 100 5 10