Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613121212_7lz1 AGAAACAAACACCGTAACTCATACGTTGGTTG(+AGGACA)GATCCTTTTGTACCTGGTTAGTGAGAAACTT NGG 10 8 17 8 24 0 100 5 10
    240613121211_zqch TTCTATTAAAACCTGTCCCTGGTACTATCATG(+AGGACA)GTACCAAGCTGTGTAAAACACTGCACTGAAT NGG 10 8 17 8 24 0 100 5 10
    240613121210_5npr AACAAATTCAGTGCAGTGTTTTACACAGCTTG(+AGGACA)GTACCATGATAGTACCAGGGACAGGTTTTAA NGG 10 8 17 8 24 0 100 5 10
    240613121209_bvo3 AGGGGTTACCAGTATTCGTTGCTCTTGCAGAG(+AGGACA)GATCCAGGTTTGCTCCTAGCACTCACACAGT NGG 10 8 17 8 24 0 100 5 10
    240613121207_4npo GAGCTACTGTGTGAGTGCTAGGAGCAAACCTG(+AGGACA)GATCCTCTGCAAGAGCAACGAATACTGGTAA NGG 10 8 17 8 24 0 100 5 10
    240613121205_ao44 TACAGTTGTGCAACTAAGTCTGGTTGTGCCTG(+AGGACA)GATCCAACTGCTACCAGCATTAAAAACACTT NGG 10 8 17 8 24 0 100 5 10
    240613121204_xvac AACCCAAGTGTTTTTAATGCTGGTAGCAGTTG(+AGGACA)GATCCAGGCACAACCAGACTTAGTTGCACAA NGG 10 8 17 8 24 0 100 5 10
    240613121202_dytb AAACCCTTTGTTCCTGCTGACTCCACCCACAG(+AGGACA)GATCCATGCAGATCTCACAGAATGCTGAGAT NGG 10 8 17 8 24 0 100 5 10
    240613121200_hvp3 GAAGCATCTCAGCATTCTGTGAGATCTGCATG(+AGGACA)GATCCTGTGGGTGGAGTCAGCAGGAACAAAG NGG 10 8 17 8 24 0 100 5 10
    240613121159_w4n3 CCAAAAGACGGAAACTTGGACTTGTGTGATGG(+AGGACA)GTACCTGCTGACCCACATGTCCACACTTTAC NGG 10 8 17 8 24 0 100 5 10