Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613121311_0as8 AGGTTCATGGGTTAGGAATGGCTTTGGCTGAG(+AGGACA)GTACCACCTCTGTGGAGCTACTGGAGAGGAG NGG 10 8 17 8 24 0 100 5 10
    240613121310_8yvl AAAAAGCTTTATTAAAAGTTAGCCCTTACAAG(+AGGACA)GATCCTATTAAAATATACACATTTTAAATAA NGG 10 8 17 8 24 0 100 5 10
    240613121309_86ta TGCATTTATTTAAAATGTGTATATTTTAATAG(+AGGACA)GATCCTTGTAAGGGCTAACTTTTAATAAAGC NGG 10 8 17 8 24 0 100 5 10
    240613121307_g2f1 TCTAATCTCTGTCAAAAGGGAGGTTAGGAAAG(+AGGACA)GTACCCCCTCCCTTGTAAACACTGAAATACA NGG 10 8 17 8 24 0 100 5 10
    240613121305_ywj5 AGATCTGTATTTCAGTGTTTACAAGGGAGGGG(+AGGACA)GTACCTTTCCTAACCTCCCTTTTGACAGAGA NGG 10 8 17 8 24 0 100 5 10
    240613121304_uwnv CTGCCAGAGGCTTGCCCAAGAAAAGGAGTCGG(+AGGACA)GTACCTGTCAGATGCTACCTGGAGGGTCAGG NGG 10 8 17 8 24 0 100 5 10
    240613121302_dq86 GATGACCTGACCCTCCAGGTAGCATCTGACAG(+AGGACA)GTACCCGACTCCTTTTCTTGGGCAAGCCTCT NGG 10 8 17 8 24 0 100 5 10
    240613121301_o7bi AGGTTGGGATGTCCCTGTTGCTAGCCCATTAG(+AGGACA)GATCCTAGCTCCAATTCTGACCTTGAAAAAG NGG 10 8 17 8 24 0 100 5 10
    240613121300_c7rd CTTGACTTTTTCAAGGTCAGAATTGGAGCTAG(+AGGACA)GATCCTAATGGGCTAGCAACAGGGACATCCC NGG 10 8 17 8 24 0 100 5 10
    240613121259_q2tk CTCCATGATGGTGGGACACCTGCTCCCGTGTG(+AGGACA)GATCCTATAGCTAGTGTCCTTCAGTCCACTG NGG 10 8 17 8 24 0 100 5 10