Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613121345_6d79 TGTGGAGTTACTTCTACTTCTCTCCTACCAGG(+AGGACA)GATCCAACTCACATTGTCAAGCAATCACAGC NGG 10 8 17 8 24 0 100 5 10
    240613121342_clw8 GCTACCCTCCCTTACTGCCATCCACACCCATG(+AGGACA)GTACCCATGGAAACATAGCCACCCTCCCCTC NGG 10 8 17 8 24 0 100 5 10
    240613121340_znhi GGCAGGAGGGGAGGGTGGCTATGTTTCCATGG(+AGGACA)GTACCATGGGTGTGGATGGCAGTAAGGGAGG NGG 10 8 17 8 24 0 100 5 10
    240613121339_oyxa GAATATTAGATATTTTTTCCATGGGGTCAAAG(+AGGACA)GTACCTAAGTATATGATTGCGAGTGGAAACA NGG 10 8 17 8 24 0 100 5 10
    240613121337_ljkc CCCTATGTTTCCACTCGCAATCATATACTTAG(+AGGACA)GTACCTTTGACCCCATGGAAAAAATATCTAA NGG 10 8 17 8 24 0 100 5 10
    240613121336_fjva GTGGGTGGATACTGAACAGAAGCAGACAGAGG(+AGGACA)GTACCATTCTGTAACGCCCTGTTTATACACC NGG 10 8 17 8 24 0 100 5 10
    240613121334_u4ap CCTTTGGTGTATAAACAGGGCGTTACAGAATG(+AGGACA)GTACCCTCTGTCTGCTTCTGTTCAGTATCCA NGG 10 8 17 8 24 0 100 5 10
    240613121333_sqmz GGCCCAGTCCCAGGAACTGTGTCTGTGCATAG(+AGGACA)GATCCGGCTCTGCCCCGGGCCCGTCAATGAA NGG 10 8 17 8 24 0 100 5 10
    240613121331_un2r CCCTCTTCATTGACGGGCCCGGGGCAGAGCCG(+AGGACA)GATCCTATGCACAGACACAGTTCCTGGGACT NGG 10 8 17 8 24 0 100 5 10
    240613121330_ypa6 GGCCTGGCTTTCGCAGGAGGGTCTGGCAGCTG(+AGGACA)GATCCATGTCTGTCCACCCACGCGGGGGAAC NGG 10 8 17 8 24 0 100 5 10