Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613121436_p51h ACTTAGTTTTATAAGCTAAGAACTTTTTTGGG(+AGGACA)GATCCTTGTATCTCAGCTCAGTTTTACTTGA NGG 10 8 17 8 24 0 100 5 10
    240613121435_0vhe AGTAGCCATTCTCTAAATGAACTGTAGGAGTG(+AGGACA)GATCCTTAGGGAAGGGAGCGCAGGTCATTCA NGG 10 8 17 8 24 0 100 5 10
    240613121433_adnf AGTTCTGAATGACCTGCGCTCCCTTCCCTAAG(+AGGACA)GATCCACTCCTACAGTTCATTTAGAGAATGG NGG 10 8 17 8 24 0 100 5 10
    240613121431_o6fu ACAAGAAACCCCTGTCTAACGCAGGGCCTCAG(+AGGACA)GATCCGAGACGGCTTTGGAAGTGAACTGCAA NGG 10 8 17 8 24 0 100 5 10
    240613121430_1s3a TGGCATTGCAGTTCACTTCCAAAGCCGTCTCG(+AGGACA)GATCCTGAGGCCCTGCGTTAGACAGGGGTTT NGG 10 8 17 8 24 0 100 5 10
    240613121428_a28x ACAAGAAACCCCTGTCTAACGCAGGGCCTCAG(+AGGACA)GATCCGAGACGGCTTTGGAAGTGAACTGCAA NGG 10 8 17 8 24 0 100 5 10
    240613121427_zemw TGGCATTGCAGTTCACTTCCAAAGCCGTCTCG(+AGGACA)GATCCTGAGGCCCTGCGTTAGACAGGGGTTT NGG 10 8 17 8 24 0 100 5 10
    240613121425_n6d1 GGATGGACCATCTAGTGATTGCCATATGCAGG(+AGGACA)GATCCATCCCATAATCAGCTTCCAAATGCTG NGG 10 8 17 8 24 0 100 5 10
    240613121424_4pbp GGTGTCAGCATTTGGAAGCTGATTATGGGATG(+AGGACA)GATCCCTGCATATGGCAATCACTAGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240613121422_imcn TAGGCTCAAGGTGATGCTGACCACATCTCCTG(+AGGACA)GTACCTTCAACTAGAATTTCTAGACCAACAA NGG 10 8 17 8 24 0 100 5 10