Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613121328_hruj AGGAAGTTCCCCCGCGTGGGTGGACAGACATG(+AGGACA)GATCCAGCTGCCAGACCCTCCTGCGAAAGCC NGG 10 8 17 8 24 0 100 5 10
    240613121327_m24c GGGACCATCTCCTGAAGCCTCAGCACTGCCAG(+AGGACA)GATCCACATTCCTGACCTGGATACTGGCTGA NGG 10 8 17 8 24 0 100 5 10
    240613121325_yzjf ACTTTTCAGCCAGTATCCAGGTCAGGAATGTG(+AGGACA)GATCCTGGCAGTGCTGAGGCTTCAGGAGATG NGG 10 8 17 8 24 0 100 5 10
    240613121324_ko66 TGTAGCCTTGCCCTGTCTTGGGCCTGGTGGAG(+AGGACA)GATCCTGGAGCCTCTTGGTGGTGCATTGTCC NGG 10 8 17 8 24 0 100 5 10
    240613121322_tl5k AGAAAGGACAATGCACCACCAAGAGGCTCCAG(+AGGACA)GATCCTCCACCAGGCCCAAGACAGGGCAAGG NGG 10 8 17 8 24 0 100 5 10
    240613121319_w48e ATTTGGCAACCTAACCAGGCAGCTGCCCCTGG(+AGGACA)GATCCCTAGCTTCTCCAACCTGAGCACCAGC NGG 10 8 17 8 24 0 100 5 10
    240613121318_sr33 GGAATGCTGGTGCTCAGGTTGGAGAAGCTAGG(+AGGACA)GATCCCAGGGGCAGCTGCCTGGTTAGGTTGC NGG 10 8 17 8 24 0 100 5 10
    240613121317_1ys9 GAGCCCTTTATTGGCTTGGATTAGCTGTGTAG(+AGGACA)GATCCAGGGACTGTCCTCGTCCTCGGGTTGG NGG 10 8 17 8 24 0 100 5 10
    240613121314_9xdn GCAAGCCAACCCGAGGACGAGGACAGTCCCTG(+AGGACA)GATCCTACACAGCTAATCCAAGCCAATAAAG NGG 10 8 17 8 24 0 100 5 10
    240613121313_152t AATGTCTCCTCTCCAGTAGCTCCACAGAGGTG(+AGGACA)GTACCTCAGCCAAAGCCATTCCTAACCCATG NGG 10 8 17 8 24 0 100 5 10