Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613121226_3rga TATTTTTATTTATTTATATTTTACAGTCCTGG(+AGGACA)GATCCATCCGAGGGTCTCTTGACCGACAGGC NGG 10 8 17 8 24 0 100 5 10
    240613121225_w3g2 CAATGCTGTTCGCTAGATTTTGCAACCACCTG(+AGGACA)GATCCTGTTTGCCGATACCCCTCATCCGGAT NGG 10 8 17 8 24 0 100 5 10
    240613121223_hpte CAGAAATCCGGATGAGGGGTATCGGCAAACAG(+AGGACA)GATCCAGGTGGTTGCAAAATCTAGCGAACAG NGG 10 8 17 8 24 0 100 5 10
    240613121222_j9p1 CTGTGTCTCATATTTATATCATAAAAACATTG(+AGGACA)GATCCCTATAACTTCAAAGTCGTGTGCTTCT NGG 10 8 17 8 24 0 100 5 10
    240613121220_t6st AGTGAAGAAGCACACGACTTTGAAGTTATAGG(+AGGACA)GATCCAATGTTTTTATGATATAAATATGAGA NGG 10 8 17 8 24 0 100 5 10
    240613121219_1crp AGGTTTCTAATTGCCCAGCTGCTAGGTAACTG(+AGGACA)GTACCTTTTAAAGCCCCCTGTAATTTTCCTT NGG 10 8 17 8 24 0 100 5 10
    240613121218_irar GAGGGAAGGAAAATTACAGGGGGCTTTAAAAG(+AGGACA)GTACCAGTTACCTAGCAGCTGGGCAATTAGA NGG 10 8 17 8 24 0 100 5 10
    240613121216_ki5h ATGAAAATGCCAAAATTAATCCCTTTTAGTTG(+AGGACA)GATCCTATTCTATGTTGACCTTTTGTAATCA NGG 10 8 17 8 24 0 100 5 10
    240613121215_949k GTTATTGATTACAAAAGGTCAACATAGAATAG(+AGGACA)GATCCAACTAAAAGGGATTAATTTTGGCATT NGG 10 8 17 8 24 0 100 5 10
    240613121214_frbg AGACCAAGTTTCTCACTAACCAGGTACAAAAG(+AGGACA)GATCCAACCAACGTATGAGTTACGGTGTTTG NGG 10 8 17 8 24 0 100 5 10