Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240903072205_srr1 TGAACGGTGGAAGGCGGCAGGCCGAGGCTTTTCCGCCCGCTGAAAGTCAGCGAGAAAAACAGCGCGCGGGGAGCAAAAGCACGGCGCCTACGCCCTTCTC(+AGTT)AGGGTTAGACAAAAAATGGCCACCACCCCTCCCAGGCCCACCCTCCGCAACCCGGTGCGCTGCCGGGCGAGTCGGCTTATAAAGGGAGCGGCCGCCGACC NGG 10 8 17 8 24 0 100 5 10
    240830165619_prf0 CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240830083203_6f7c TGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCCG(/A)CTTGGCGAGGCAGAGACTGCTGGGCCGGTCATGGAGCGTGCCAGTCATCCGCCAC NGG 10 8 17 8 24 0 100 5 10
    240829112226_143f AATGGTGCCATTCCTGATCAAGTGTGTAATAATCCCCAGTGTGGACAACCTTTCCATGAAA(+TAT)GTCTGTATGAGGTAAAACTAGTAATTCTCTACATAGGAAATACAGCATTAAAA NG 10 8 17 8 24 0 100 5 50
    240829112014_2nvv AATGGTGCCATTCCTGATCAAGTGTGTAATAATCCCCAGTGTGGACAACCTTTCCATGAAA(+TAT)GTCTGTATGAGGTAAAACTAGTAATTCTCTACATAGGAAATACAGCATTAAAA NG 10 8 17 8 24 0 100 5 10
    240828231231_3d29 TCTGCAGCCAGGAGAGCAGGGACGTCCTGTGCGAACTGTCAGACCACCACAACCACACTCTGGAGGAGGAATGCCAATGGGG(+G)ACCCTGTCTGCAATGCCTGTGGGCTCTACTACAAGCTTCACAAT NGG 10 8 17 8 24 0 100 5 10
    240828231202_ufxy TCTGCAGCCAGGAGAGCAGGGACGTCCTGTGCGAACTGTCAGACCACCACAACCACACTCTGGAGGAGGAATGCCAATGGGG(+G)ACCCTGTCTGCAATGCCTGTGGGCTCTACTACAAGCTTCACAAT NGG 10 8 17 8 24 0 100 5 10
    240827162609_iuj1 gcatgtatgtggagacaaaacactaaatcttcaagactagaagccataaaaattcaaatcctcagtaaacttcgcctggaaacagctcctaacattagcaaagatgctataagacaacttttgcccaaagctcctccactccgggaactgattgatcagtacgatgtccagagagatgacagcagtgatggctccttggaagatgatgattatcacgctacgacg(+TA)gaaacgatcattaccatgcctacagagtgtaagtagtcctattagtgtatatcaacaattctgctgactgttgttccagtgtttatgagaaacagatctattttcaggctcttttaacaagctgttggcttgtCC NGG 10 8 17 8 24 0 100 5 10
    240827160755_w4zz ttgcccaaagctcctccactccgggaactgattgatcagtacgatgtccagagagatgacagcagtgatggctccttggaagatgatgattatcacgctacgacgTAgaaacgatcattaccatgc(+T)ctacagagtgtaagtagtcctattagtgtatatcaacaattctgctgactgttgttccagtgtttatgagaaacagatctattttcaggctcttttaacaagctgttggcttg NGG 10 8 17 8 24 0 100 5 10
    240827160710_4b44 ataaaaattcaaatcctcagtaaacttcgcctggaaacagctcctaacattagcaaagatgctataagacaacttttgcccaaagctcctccactccgggaactgattgatcagtacgatgtccagagagatgacagcagtgatggctccttggaagatgatgattatcacgctacgacgTAgaaacgatcattaccatgc(+T)ctacagagtgtaagtagtcctattagtgtatatcaacaattctgctgactgttgttccagtgtttatgagaaacagatctattttcaggctcttttaacaagctgttggcttgtacgtaagtaggagggaaaagagtttcttttttcaagatttcatgagaaataaactaatgagactgaaagctgctgtattattgttttccttagctaaacagctgaaaataa NGG 10 8 17 8 24 0 100 5 10