Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240624195343_g574 TGGCCTGGCTGGAAAAAGGTGTAAATACCTCCGTCATGCTGGAAAATCTCCGCCAAAGCTTATCCCTTCCCTCGATGCTTCGGGATGCTGCAATTGGCACTACCCCTTTCTCTACTTGCTCGGTGGGGACTTGGTTTACTCCTTCAGCACCACAGGAAAAGAGTACAAACACATCCCAGACAGGCCTGGTTGGCACCAAGC(/G)ACAGTACTTCTGAGACAGAGCAGCTCCTGTGTGGGTAAGCGCCCCTATGTCCTTGCACCTCGTGAGTTCCATTACCCTGGACCTTCCCCTCCCATCCCTTCCCCTCCTTTTCTTTTCGTCATGTTCTTCTCTTTCTGCTGCAGCCGGCCTCCAGATCTGACTGCCTTGTCTCGACATGACTTGGAAGATAACCTGCTGA NGG 10 8 17 8 24 0 100 5 10
    240623214806_gxcp taatctctatatgtctttctctaaccaacttatttctactcatccctgccaccagtgcgtctgtaatacttattaacttgtaatatattacataaggtaagctccatgagggtaga(/t)aattttgttctttctattttctgtttttcctccagcccctggaacaatacctgacacagagtaa NGG 10 8 17 8 50 0 100 5 50
    240623214633_kro3 taatctctatatgtctttctctaaccaacttatttctactcatccctgccaccagtgcgtctgtaatacttattaacttgtaatatattacataaggtaagctccatgagggtaga(/t)aattttgttctttctattttctgtttttcctccagcccctggaacaatacctgacacagagtaa NGG 10 8 17 8 40 0 100 5 10
    240623212143_uk4c taatctctatatgtctttctctaaccaacttatttctactcatccctgccaccagtgcgtctgtaatacttattaacttgtaatatattacataaggtaagctccatgagggtaga(/t)aattttgttctttctattttctgtttttcctccagcccctggaacaatacctgacacagagtaagcactcaataaataattggtgaaggcattaatcccagaggcagttcagcaagaggctgat NGG 10 8 17 8 24 0 100 5 10
    240622013134_1ept CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC NGG 10 8 17 8 24 0 100 5 10
    240622004233_iooo CCTATGGGATGCAGCAGGGAATACTGATCCTGATTTAACAGTGATAATAACTTTTCACTTA(/G)GGGCCTACAGTACTGCTTATCAGAGAAGCCAAAGCTTCTCCCCCTGGAGCTGGAGAAGACAGCCATCCAAAATTACACTGTCACGGAGTTCCAGCCCCTCTATTACGTGGCAGAGAGTTTTAATGATGCCAAGGAGAAAGTAAGGTGAG NGG 10 8 17 8 30 0 100 5 10
    240622003812_jasr CCTATGGGATGCAGCAGGGAATACTGATCCTGATTTAACAGTGATAATAACTTTTCACTTA(/G)GGGCCTACAGTACTGCTTATCAGAGAAGCCAAAGCTTCTCCCCCTGGAGCTGGAGAAGACAGCCATCCAAAATTACACTGTCACGGAGTTCCAGCCCCTCTATTACGTGGCAGAGAGTTTTAATGATGCCAAGGAGAAAGTAAGGTGAG NGG 10 8 17 20 20 0 100 5 10
    240622003712_9584 CCTATGGGATGCAGCAGGGAATACTGATCCTGATTTAACAGTGATAATAACTTTTCACTTA(/G)GGGCCTACAGTACTGCTTATCAGAGAAGCCAAAGCTTCTCCCCCTGGAGCTGGAGAAGACAGCCATCCAAAATTACACTGTCACGGAGTTCCAGCCCCTCTATTACGTGGCAGAGAGTTTTAATGATGCCAAGGAGAAAGTAAGGTGAG NGG 10 8 17 8 24 0 100 5 10
    240622001454_opfw TGAGCTATGAAGGGTAGAACCTAGTATGTGCAAAGTAATTTGGCTCACAGCATTTGGGCTGTGATGTAGAAGGAATCGGGGTGAGATGAGAGAAGGGGCACAAATGGCCTATGGGATGCAGCAGGGAATACTGATCCTGATTTAACAGTGATAATAACTTTTCACTTG(/A)GGGCCTACAGTACTGCTTATCAGAGAAGCCAAAGCTTCTCCCCCTGGAGCTGGAGAAGACAGCCATCCAAAATTACACTGTCACGGAGTTCCAGCCCCTCTATTACGTGGCAGAGAGTTTTAATGATGCCAAGGAGAAAGTAAGGTGAG NGG 10 8 17 8 24 0 100 5 10
    240622001442_4u2a TGAGCTATGAAGGGTAGAACCTAGTATGTGCAAAGTAATTTGGCTCACAGCATTTGGGCTGTGATGTAGAAGGAATCGGGGTGAGATGAGAGAAGGGGCACAAATGGCCTATGGGATGCAGCAGGGAATACTGATCCTGATTTAACAGTGATAATAACTTTTCACTTG(/T)GGGCCTACAGTACTGCTTATCAGAGAAGCCAAAGCTTCTCCCCCTGGAGCTGGAGAAGACAGCCATCCAAAATTACACTGTCACGGAGTTCCAGCCCCTCTATTACGTGGCAGAGAGTTTTAATGATGCCAAGGAGAAAGTAAGGTGAG NGG 10 8 17 8 24 0 100 5 10