Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240627223810_cnfe CTTTCCCGCAGCCCCCCAGCCTATACAGCCCCAGATATTCTTACCACAACATTGCTGACGTGTGTGAGCTGGCCCTGGGCATGGCCGGCACCACAGCCACTTCGCTGGACCAAACAGACCAACATG(-C)TTTAAAAGCCTCAAAAACCATTGAAACTGGCAGGGAAGGAAAGGCAACCAAGCAGAGGGAGTCAGAAGGCTGCAGTTCCAAGCCTGACCAAGACACGGTGTGGCCACGACCCCA NGG 100 9 20 8 35 1 100 5 10
    240626065620_kwl8 TTGACGCGGTTCTATCTAGTTACGCGTTAAACCAACTAGAAAgg(-TAATTTCTcCTaTTGgAGATACTGGTATTGCGGAGCCACTTTTT)tagctTAATTTCTcCTaTTGgAGAT NGG 10 8 17 8 24 0 100 5 10
    240626065556_30b7 TTGACGCGGTTCTATCTAGTTACGCGTTAAACCAACTAGAAAggT(-AATTTCTcCTaTTGgAGATACTGGTATTGCGGAGCCACTTTTT)agctTAATTTCTcCTaTTGgAGAT NGG 10 8 17 8 24 0 100 5 10
    240626065517_wmrb TTGACGCGGTTCTATCTAGTTACGCGTTAAACCAACTAGAAAggT(-AATTTCTcCTaTTGgAGATACTGGTATTGCGGAGCCACTTTTT)cagctTAATTTCTcCTaTTGgAGAT NGG 10 8 17 8 24 0 100 5 10
    240626065446_b1y5 TTGACGCGGTTCTATCTAGTTACGCGTTAAACCAACTAGAAA(-ggTAATTTCTcCTaTTGgAGATACTGGTATTGCGGAGCCACTTTTT)cagctTAATTTCTcCTaTTGgAGAT NGG 10 8 17 8 24 0 100 5 10
    240626065435_ku35 TTGACGCGGTTCTATCTAGTTACGCGTTAAACCAACTAGAAA(-ggTAATTTCTcCTaTTGgAGATACTGGTATTGCGGAGCCACTTTTT)caccTAATTTCTcCTaTTGgAGAT NGG 10 8 17 8 24 0 100 5 10
    240626065424_mora TTGACGCGGTTCTATCTAGTTACGCGTTAAACCAACTAGAAA(-ggTAATTTCTcCTaTTGgAGATACTGGTATTGCGGAGCCACTTTTT)ccTAATTTCTcCTaTTGgAGAT NGG 10 8 17 8 24 0 100 5 10
    240626065409_hpez TTGACGCGGTTCTATCTAGTTACGCGTTAAACCAACTAGAAA(-ggTAATTTCTcCTaTTGgAGATACTGGTATTGCGGAGCCACTTTTT)ggacgtTAATTTCTcCTaTTGgAGAT NGG 10 8 17 8 24 0 100 5 10
    240626065349_3s1h TTGACGCGGTTCTATCTAGTTACGCGTTAAACCAACTAGAAAggTAATTTCTcCTaTTGgAGAT(-ACTGGTATTGCGGAGCCACTTTTT)TcCTaTTGgAGAT NGG 10 8 17 8 24 0 100 5 10
    240626065044_0wm6 TTGACGCGGTTCTATCTAGTTACGCGTTAAACCAACTAGAAAgg(-TAATTTCTcCTaTTGgAGATACTGGTATTGCGGAGCCACGTTTTT)acTAATTTCTcCTaTTGgAGAT NGG 10 8 17 8 24 0 100 5 10