Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240704091056_2kiw ACAACCACAGAACCACAAGTTGGGTAGCCTGGCAGTGTCAGAAGTCTGAACCCAGCATAGTGGTCAGCAGGCAGGACGAATCACACTGAATGCAAACCACAGGGTTTCGCAGCGTGGTGAGCATCACCAACCCACAGCCAAGGCGGCGCTGGCTTTTTTTTTTTTTTTAATCTTTAACAATTTGAATATTTGTTTTTACAAAGGTGCATTTTTTAATAGGGCTTGGGGAGTCCCAGAGGTATCCAGCAGAGGGGAGAAGAAAGAGAGATGTAGGGCTAGAGGGGTGAGGCTGAAACAGTGACCTGTCTTGGTTTTCGCTCCGAAGGTAAAAGAAATCATTGAGTCCCCCGCCTTCAGAAGAGGGTGCATTTTCAGGAGGAAGCGATGGCTTCAGACAGCATATTTGAGTCATTTCCTTCG(+ctt)TACCCACAGTGCTTCATGAGAGGTGAGTACATGCTGGTCTTGTAATATCTACTTTTGCTCAGCTTTGCCTGTAATGAAATGGCAGCTTGTTTCACCTCGGTGCAGAGATGCCTCGGTGCCTGCCAGTTCCCTGTCTTGTTTGTGAGAGGAATTCAAACTGAGGCATATGATTACAAGTCTATTGGATTACTTACTAATCAGATGGAAGCTCTTCAGAAATGTTTTAATAAATACTTAGTTATGCTGTTGGAGTGTTCAGTCGGTGCGTGAGAACTTTGTCAAGTGCGAGTAAGTTGTGCTGGTCTTGTTTAAAGGCTTAGAGATTATGAAGCTTGCACGAAGCTCCCTGGGTTTTATTTTGCTGTCCCTTGCCCGGAATG NGG 10 8 17 8 24 0 100 5 10
    240704090658_jewr ACAACCACAGAACCACAAGTTGGGTAGCCTGGCAGTGTCAGAAGTCTGAACCCAGCATAGTGGTCAGCAGGCAGGACGAATCACACTGAATGCAAACCACAGGGTTTCGCAGCGTGGTGAGCATCACCAACCCACAGCCAAGGCGGCGCTGGCTTTTTTTTTTTTTTTAATCTTTAACAATTTGAATATTTGTTTTTACAAAGGTGCATTTTTTAATAGGGCTTGGGGAGTCCCAGAGGTATCCAGCAGAGGGGAGAAGAAAGAGAGATGTAGGGCTAGAGGGGTGAGGCTGAAACAGTGACCTGTCTTGGTTTTCGCTCCGAAGGTAAAAGAAATCATTGAGTCCCCCGCCTTCAGAAGAGGGTGCATTTTCAGGAGGAAGCGATGGCTTCAGACAGCATATTTGAGTCATTTCCTTCGTACCCACAG(+ctt)TGCTTCATGAGAGGTGAGTACATGCTGGTCTTGTAATATCTACTTTTGCTCAGCTTTGCCTGTAATGAAATGGCAGCTTGTTTCACCTCGGTGCAGAGATGCCTCGGTGCCTGCCAGTTCCCTGTCTTGTTTGTGAGAGGAATTCAAACTGAGGCATATGATTACAAGTCTATTGGATTACTTACTAATCAGATGGAAGCTCTTCAGAAATGTTTTAATAAATACTTAGTTATGCTGTTGGAGTGTTCAGTCGGTGCGTGAGAACTTTGTCAAGTGCGAGTAAGTTGTGCTGGTCTTGTTTAAAGGCTTAGAGATTATGAAGCTTGCACGAAGCTCCCTGGGTTTTATTTTGCTGTCCCTTGCCCGGAATG NGG 10 8 17 8 24 0 100 5 10
    240704090605_820k CCAAAGAGGGGAACCCCTGAGGGTGTCCCCAAGCTTGTTCTATTTCAGAGGGCTGATCCCCTTGCTCCCTCAAGCTCCCAGTCAATGGGCTTGCCAGCACCCCGGCCCCCACCCATTTTGGCGCCAACAGCTAAGGGCACGCGCGACAGGAAGCACAGCCCGCGCCAAACGCTGCCCCGCGCATGCGCGCCCTGGCTGTCAGCGGCCGCAGGGCCACCCGGAGATACCCCCAATATATGCCTCGGCATCGGTCCCGCCCCTCACCCCCACCCTGCGTGGCACCTACCGCCTGCGGACATGGTCCGGGAGCGAGCCTGCCGGGGAGGCAAGCGCAGGCACTCGGGCTGGAGCTGTTCGCGCTGGCATCTTGCAGGTTGCAGACGACAGAACAGCTCTGAACGAGACCCCGGCT(+ctt)TTTTCGCGCGCGCGGAAACCAATTGGGAGGGGGCGGCGCAAGCGGAAGCAGCATGTACCACACAGGGCAAGAGAGTGGGGGAAGACCATGCTATGCCCGAAGGGAGTGCGCATGCGCGCAACGGAGGCACCCACACCTCCTGGCTATAGTGGGCTCGTCCGTGCTGCGACTGGCGCCACCCACCTCCCCCTTGCCTGAAGAGGTGGCTCGGCACTTGAGAGCAGGTAGTGCTGCTCTTGCAGAGGATGACTCTGGTTTGCTTGCCAGCACCCGCATCCAGCCGCTCCAAGCCCCTGTAATTCCACTTCCAGGGGGTCCTACAGCCTCTTCTGGCCTCTATGGGCATTGCACTCACATGCACAAACACAACTTTAAAAAAAAAAAAATC NGG 10 8 17 8 24 0 100 5 10
    240704090538_jmiv CGGGTAGGTTTGAATCATCACGCAGGCCCTGGCCTCCACCCGCCCCCACCAGCCCCCTGGCCTCAGTTCCCTGGCAACATCTGGGGTTGGGGGGGCAGCAGGAACAAGGGCCTCTGTCTGCCCAGCTGCCTCCCCCTTTGGGTTTTGCCAGACTCCACAGTGCATACGTGGGCTCCAACAGGTCCTCTTCCCTCCCAGTCACTGACTAACCCCGGAACCACACAGCTTCCCGTTCTCAGCTCCACAAACTTGGTGCCAAATTCTTCTCCCCTGGGAAGCATCCCTGGACACTTCCCAAAGGACCCCAGTCACTCCAGCCTGTTGGCTGCCGCTCACTTTGATGTCTGCAGGCCAGATGAGGGCTCCAGATGGCACATTGTCAGAGGGACACACTGTGGCCCCTGTG(+ctt)CCCAGCCCTGGGCTCTCTGTACATGAAGCAACTCCAGTCCCAAATATGTAGCTGTTTGGGAGGTCAGAAATAGGGGGTCCAGGAGCAAACTCCCCCCACCCCCTTTCCAAAGCCCATTCCCTCTTTAGCCAGAGCCGGGGTGTGCAGACGGCAGTCACTAGGGGGCGCTCGGCCACCACAGGGAAGCTGGGTGAATGGAGCGAGCAGCGTCTTCGAGAGTGAGGACGTGTGTGTCTGTGTGGGTGAGTGAGTGTGTGCGTGTGGGGTTGAGGGCGTTGGAGCGGGGAGAAGGCCAGGGGTCACTCCAGGATTCCAATAGATCTGTGTGTCCCTCTCCCCACCCGTCCCTGTCCGGCTCTCCGCCTTCCCCTGCCCCCTTCAATATTCCTAGCAA NGG 10 8 17 8 24 0 100 5 10
    240704090505_mzil ACAACCACAGAACCACAAGTTGGGTAGCCTGGCAGTGTCAGAAGTCTGAACCCAGCATAGTGGTCAGCAGGCAGGACGAATCACACTGAATGCAAACCACAGGGTTTCGCAGCGTGGTGAGCATCACCAACCCACAGCCAAGGCGGCGCTGGCTTTTTTTTTTTTTTTAATCTTTAACAATTTGAATATTTGTTTTTACAAAGGTGCATTTTTTAATAGGGCTTGGGGAGTCCCAGAGGTATCCAGCAGAGGGGAGAAGAAAGAGAGATGTAGGGCTAGAGGGGTGAGGCTGAAACAGTGACCTGTCTTGGTTTTCGCTCCGAAGGTAAAAGAAATCATTGAGTCCCCCGCCTTCAGAAGAGGGTGCATTTTCAGGAGGAAGCGATGGCTTCAGACAGCATATTTGAGTCATTTCCTTCG(+ctt)TACCCACAGTGCTTCATGAGAGGTGAGTACATGCTGGTCTTGTAATATCTACTTTTGCTCAGCTTTGCCTGTAATGAAATGGCAGCTTGTTTCACCTCGGTGCAGAGATGCCTCGGTGCCTGCCAGTTCCCTGTCTTGTTTGTGAGAGGAATTCAAACTGAGGCATATGATTACAAGTCTATTGGATTACTTACTAATCAGATGGAAGCTCTTCAGAAATGTTTTAATAAATACTTAGTTATGCTGTTGGAGTGTTCAGTCGGTGCGTGAGAACTTTGTCAAGTGCGAGTAAGTTGTGCTGGTCTTGTTTAAAGGCTTAGAGATTATGAAGCTTGCACGAAGCTCCCTGGGTTTTATTTTGCTGTCCCTTGCCCGGAATG NGG 10 8 17 8 24 0 100 5 10
    240703163036_7isv GTGCCCCGCCTTAGAGGTGGGGCCTTATTTGATTGCCAAGTAATATTCCCCAATGGAGTACTAGCTCATGGTGACGGGCAGGCAGCTTGACTAATGAGTCCTCGGCGTGGCCGGCGCAGCTCTCCAATCGCCGGGCGGCGGGCCCCAGTCTGAGCGGCGATGGCGGCGGCGGCGGCGGCGGCAGCAGCAGCG(+GCG)GGGGCTGCGGGCGGTCGGGGCTCCGGGCCGGGGCGGCGGCGCCATCTTGTGCCCGGGGCCGGTGGGGAGGCCGGGGAGGGGGCCCCGGGGGGCGCAGGGGACTACGGGAACGGCCTGGAGTCTGAGGAACTGGAGCCTGAGGAGCTGCTGCTGGAGCCCGAGCCGGAGCCCGAGCCCGAAGAGGAGCCGCCCCGGCCCCGCGCCCCCCCGGGAGCTCCGGGCCCTGGGCCTGGTTCGGGAGCCCCCGGC NGG 10 8 17 10 35 0 100 5 25
    240703162446_bqcr GTGCCCCGCCTTAGAGGTGGGGCCTTATTTGATTGCCAAGTAATATTCCCCAATGGAGTACTAGCTCATGGTGACGGGCAGGCAGCTTGACTAATGAGTCCTCGGCGTGGCCGGCGCAGCTCTCCAATCGCCGGGCGGCGGGCCCCAGTCTGAGCGGCGATG(+GCG)GCGGCGGCGGCGGCGGCGGCAGCAGCAGCGGGGGCTGCGGGCGGTCGGGGCTCCGGGCCGGGGCGGCGGCGCCATCTTGTGCCCGGGGCCGGTGGGGAGGCCGGGGAGGGGGCCCCGGGGGGCGCAGGGGACTACGGGAACGGCCTGGAGTCTGAGGAACTGGAGCCTGAGGAGCTGCTGCTGGAGCCCGAGCCGGAGCCCGAGCCCGAAGAGGAGCCGCCCCGGCCCCGCGCCCCCCCGGGAGCTCCGGGCCCTGGGCCTGGTTCGGGAGCCCCCGGC NGG 10 8 17 8 24 0 100 5 10
    240703144424_9r1l GTGGCCGGCGCAGCTCTCCAATCGCCGGGCGGCGGGCCCCAGTCTGAGCGGCGATGGCGGCGGCGGCGGCGGCGGCAGCAGCAGCG(+GCG)GGGGCTGCGGGCGGTCGGGGCTCCGGGCCGGGGCGGCGGCGCCATCTTGTGCCCGGGGCCGGTGGGGAGGCCGGGGAGGGGGCCCCGGGGGGC NGG 10 8 17 8 24 0 100 5 10
    240703121625_09oh CTGTGGTTAGTCACAGATGCAAGAGATCCAAG(+AGGACA)GATCCGAGGCTTTGTGAGCACAGTAATTAAG NGG 10 8 17 8 24 0 100 5 10
    240703121623_yiy2 TTACTCTTAATTACTGTGCTCACAAAGCCTCG(+AGGACA)GATCCTTGGATCTCTTGCATCTGTGACTAAC NGG 10 8 17 8 24 0 100 5 10