Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240704101432_1wo8 AATGGACTAAACCTCTGATACTGTAAGCCAACCCTAGTTAAATTATAAGAGTTGCTGTGGTCATGGAGTCTCTTCACTGCAATAGAACACTGACTAAGACAGGAAGGGATGAAGCCAACACGGATGCGAAGCTGGAGGAGAGGGGCATTTGAGGGAAAACTCTTGCATTTCTGCATGGAGCAATGCAGAGAGCACAGAGAGGTCAGTGCAGACTCTGGAGCCCTGAGGTGGGTTGGGAAGAGGACGGGCAGAGCAAAGCAAGTGGAGGCTGGAGGCTTACTGGGGACCTTACCAGGGGAGCGGTCTTCCTCTGTCTGGTGCCATGCTGGGATAATTCGCTCCAGGTTCCATGGGATGCTCTGGGCGAAGACAAAGGAGTCTTCCTCAATGTACTCCACATGGGGCAACTTCAGGGCCTACA(+ctt)GAACCAGACAGCCATGCACTTAGCTTCAAAAGCCCTTCAGCTAATAGCAGCCCACAGCAGCCCCACCTGACTGTCCACCTCCCCAGCCTTCACAAAGTCTTCCTCCTGGGGCCCAGAAGACAGACCAGGGGCCAGTGTCCTCATTTCTCTCTGGCTGGGGAGCACGGTGTTCCGTAACTCCAGCCCCTGAATCTATATGCCTGCTCGGAGGGTTCTCTATTCCACTCTCTGGATTCTGAGCTCCACATCTCCCCTGAGAGTCAGGTTATGCCTAGCTTCAGGGATGTGGTCAGGAATCTTAGTACCGTCAATCTCCAGTCAGGATACCAGTGCTGGCCATGGGGCCTCTGCTCCCATCCCTGGCCTTTGCACTGGCCCT NGG 10 8 17 8 24 0 100 5 10
    240704100712_w2mo CCAAAGAGGGGAACCCCTGAGGGTGTCCCCAAGCTTGTTCTATTTCAGAGGGCTGATCCCCTTGCTCCCTCAAGCTCCCAGTCAATGGGCTTGCCAGCACCCCGGCCCCCACCCATTTTGGCGCCAACAGCTAAGGGCACGCGCGACAGGAAGCACAGCCCGCGCCAAACGCTGCCCCGCGCATGCGCGCCCTGGCTGTCAGCGGCCGCAGGGCCACCCGGAGATACCCCCAATATATGCCTCGGCATCGGTCCCGCCCCTCACCCCCACCCTGCGTGGCACCTACCGCCTGCGGACATGGTCCGGGAGCGAGCCTGCCGGGGAGGCAAGCGCAGGCACTCGGGCTGGAGCTGTTCGCGCTGGCATCTTGCAGGTTGCAGACGACAGAACAGCTCTGAACGAGACCCCGGCT(+agga)TTTTCGCGCGCGCGGAAACCAATTGGGAGGGGGCGGCGCAAGCGGAAGCAGCATGTACCACACAGGGCAAGAGAGTGGGGGAAGACCATGCTATGCCCGAAGGGAGTGCGCATGCGCGCAACGGAGGCACCCACACCTCCTGGCTATAGTGGGCTCGTCCGTGCTGCGACTGGCGCCACCCACCTCCCCCTTGCCTGAAGAGGTGGCTCGGCACTTGAGAGCAGGTAGTGCTGCTCTTGCAGAGGATGACTCTGGTTTGCTTGCCAGCACCCGCATCCAGCCGCTCCAAGCCCCTGTAATTCCACTTCCAGGGGGTCCTACAGCCTCTTCTGGCCTCTATGGGCATTGCACTCACATGCACAAACACAACTTTAAAAAAAAAAAAATC NGG 10 8 17 8 24 0 100 5 10
    240704100538_o9j5 CCAAAGAGGGGAACCCCTGAGGGTGTCCCCAAGCTTGTTCTATTTCAGAGGGCTGATCCCCTTGCTCCCTCAAGCTCCCAGTCAATGGGCTTGCCAGCACCCCGGCCCCCACCCATTTTGGCGCCAACAGCTAAGGGCACGCGCGACAGGAAGCACAGCCCGCGCCAAACGCTGCCCCGCGCATGCGCGCCCTGGCTGTCAGCGGCCGCAGGGCCACCCGGAGATACCCCCAATATATGCCTCGGCATCGGTCCCGCCCCTCACCCCCACCCTGCGTGGCACCTACCGCCTGCGGACATGGTCCGGGAGCGAGCCTGCCGGGGAGGCAAGCGCAGGCACTCGGGCTGGAGCTGTTCGCGCTGGCATCTTGCAGGTTGCAGACGACAGAACAGCTCTGAACGAGACCCCGGCTT(+cgg)TTTCGCGCGCGCGGAAACCAATTGGGAGGGGGCGGCGCAAGCGGAAGCAGCATGTACCACACAGGGCAAGAGAGTGGGGGAAGACCATGCTATGCCCGAAGGGAGTGCGCATGCGCGCAACGGAGGCACCCACACCTCCTGGCTATAGTGGGCTCGTCCGTGCTGCGACTGGCGCCACCCACCTCCCCCTTGCCTGAAGAGGTGGCTCGGCACTTGAGAGCAGGTAGTGCTGCTCTTGCAGAGGATGACTCTGGTTTGCTTGCCAGCACCCGCATCCAGCCGCTCCAAGCCCCTGTAATTCCACTTCCAGGGGGTCCTACAGCCTCTTCTGGCCTCTATGGGCATTGCACTCACATGCACAAACACAACTTTAAAAAAAAAAAAATC NGG 10 8 17 8 24 0 100 5 10
    240704100525_swl2 CCAAAGAGGGGAACCCCTGAGGGTGTCCCCAAGCTTGTTCTATTTCAGAGGGCTGATCCCCTTGCTCCCTCAAGCTCCCAGTCAATGGGCTTGCCAGCACCCCGGCCCCCACCCATTTTGGCGCCAACAGCTAAGGGCACGCGCGACAGGAAGCACAGCCCGCGCCAAACGCTGCCCCGCGCATGCGCGCCCTGGCTGTCAGCGGCCGCAGGGCCACCCGGAGATACCCCCAATATATGCCTCGGCATCGGTCCCGCCCCTCACCCCCACCCTGCGTGGCACCTACCGCCTGCGGACATGGTCCGGGAGCGAGCCTGCCGGGGAGGCAAGCGCAGGCACTCGGGCTGGAGCTGTTCGCGCTGGCATCTTGCAGGTTGCAGACGACAGAACAGCTCTGAACGAGACCCCGGCT(+cgg)TTTTCGCGCGCGCGGAAACCAATTGGGAGGGGGCGGCGCAAGCGGAAGCAGCATGTACCACACAGGGCAAGAGAGTGGGGGAAGACCATGCTATGCCCGAAGGGAGTGCGCATGCGCGCAACGGAGGCACCCACACCTCCTGGCTATAGTGGGCTCGTCCGTGCTGCGACTGGCGCCACCCACCTCCCCCTTGCCTGAAGAGGTGGCTCGGCACTTGAGAGCAGGTAGTGCTGCTCTTGCAGAGGATGACTCTGGTTTGCTTGCCAGCACCCGCATCCAGCCGCTCCAAGCCCCTGTAATTCCACTTCCAGGGGGTCCTACAGCCTCTTCTGGCCTCTATGGGCATTGCACTCACATGCACAAACACAACTTTAAAAAAAAAAAAATC NGG 10 8 17 8 24 0 100 5 10
    240704100514_wn25 CCAAAGAGGGGAACCCCTGAGGGTGTCCCCAAGCTTGTTCTATTTCAGAGGGCTGATCCCCTTGCTCCCTCAAGCTCCCAGTCAATGGGCTTGCCAGCACCCCGGCCCCCACCCATTTTGGCGCCAACAGCTAAGGGCACGCGCGACAGGAAGCACAGCCCGCGCCAAACGCTGCCCCGCGCATGCGCGCCCTGGCTGTCAGCGGCCGCAGGGCCACCCGGAGATACCCCCAATATATGCCTCGGCATCGGTCCCGCCCCTCACCCCCACCCTGCGTGGCACCTACCGCCTGCGGACATGGTCCGGGAGCGAGCCTGCCGGGGAGGCAAGCGCAGGCACTCGGGCTGGAGCTGTTCGCGCTGGCATCTTGCAGGTTGCAGACGACAGAACAGCTCTGAACGAGACCCCGG(+cgg)CTTTTTCGCGCGCGCGGAAACCAATTGGGAGGGGGCGGCGCAAGCGGAAGCAGCATGTACCACACAGGGCAAGAGAGTGGGGGAAGACCATGCTATGCCCGAAGGGAGTGCGCATGCGCGCAACGGAGGCACCCACACCTCCTGGCTATAGTGGGCTCGTCCGTGCTGCGACTGGCGCCACCCACCTCCCCCTTGCCTGAAGAGGTGGCTCGGCACTTGAGAGCAGGTAGTGCTGCTCTTGCAGAGGATGACTCTGGTTTGCTTGCCAGCACCCGCATCCAGCCGCTCCAAGCCCCTGTAATTCCACTTCCAGGGGGTCCTACAGCCTCTTCTGGCCTCTATGGGCATTGCACTCACATGCACAAACACAACTTTAAAAAAAAAAAAATC NGG 10 8 17 8 24 0 100 5 10
    240704100448_5rmo CCAAAGAGGGGAACCCCTGAGGGTGTCCCCAAGCTTGTTCTATTTCAGAGGGCTGATCCCCTTGCTCCCTCAAGCTCCCAGTCAATGGGCTTGCCAGCACCCCGGCCCCCACCCATTTTGGCGCCAACAGCTAAGGGCACGCGCGACAGGAAGCACAGCCCGCGCCAAACGCTGCCCCGCGCATGCGCGCCCTGGCTGTCAGCGGCCGCAGGGCCACCCGGAGATACCCCCAATATATGCCTCGGCATCGGTCCCGCCCCTCACCCCCACCCTGCGTGGCACCTACCGCCTGCGGACATGGTCCGGGAGCGAGCCTGCCGGGGAGGCAAGCGCAGGCACTCGGGCTGGAGCTGTTCGCGCTGGCATCTTGCAGGTTGCAGACGACAGAACAGCTCTGAACGAGACCCCG(+cgg)GCTTTTTCGCGCGCGCGGAAACCAATTGGGAGGGGGCGGCGCAAGCGGAAGCAGCATGTACCACACAGGGCAAGAGAGTGGGGGAAGACCATGCTATGCCCGAAGGGAGTGCGCATGCGCGCAACGGAGGCACCCACACCTCCTGGCTATAGTGGGCTCGTCCGTGCTGCGACTGGCGCCACCCACCTCCCCCTTGCCTGAAGAGGTGGCTCGGCACTTGAGAGCAGGTAGTGCTGCTCTTGCAGAGGATGACTCTGGTTTGCTTGCCAGCACCCGCATCCAGCCGCTCCAAGCCCCTGTAATTCCACTTCCAGGGGGTCCTACAGCCTCTTCTGGCCTCTATGGGCATTGCACTCACATGCACAAACACAACTTTAAAAAAAAAAAAATC NGG 10 8 17 8 24 0 100 5 10
    240704095141_jj1r CCAAAGAGGGGAACCCCTGAGGGTGTCCCCAAGCTTGTTCTATTTCAGAGGGCTGATCCCCTTGCTCCCTCAAGCTCCCAGTCAATGGGCTTGCCAGCACCCCGGCCCCCACCCATTTTGGCGCCAACAGCTAAGGGCACGCGCGACAGGAAGCACAGCCCGCGCCAAACGCTGCCCCGCGCATGCGCGCCCTGGCTGTCAGCGGCCGCAGGGCCACCCGGAGATACCCCCAATATATGCCTCGGCATCGGTCCCGCCCCTCACCCCCACCCTGCGTGGCACCTACCGCCTGCGGACATGGTCCGGGAGCGAGCCTGCCGGGGAGGCAAGCGCAGGCACTCGGGCTGGAGCTGTTCG(+ctt)CGCTGGCATCTTGCAGGTTGCAGACGACAGAACAGCTCTGAACGAGACCCCGGCTTTTTCGCGCGCGCGGAAACCAATTGGGAGGGGGCGGCGCAAGCGGAAGCAGCATGTACCACACAGGGCAAGAGAGTGGGGGAAGACCATGCTATGCCCGAAGGGAGTGCGCATGCGCGCAACGGAGGCACCCACACCTCCTGGCTATAGTGGGCTCGTCCGTGCTGCGACTGGCGCCACCCACCTCCCCCTTGCCTGAAGAGGTGGCTCGGCACTTGAGAGCAGGTAGTGCTGCTCTTGCAGAGGATGACTCTGGTTTGCTTGCCAGCACCCGCATCCAGCCGCTCCAAGCCCCTGTAATTCCACTTCCAGGGGGTCCTACAGCCTCTTCTGGCCTCTATGGGCATTGCACTCACATGCACAAACACAACTTTAAAAAAAAAAAAATC NGG 10 8 17 8 24 0 100 5 10
    240704092942_1eoh CCAAAGAGGGGAACCCCTGAGGGTGTCCCCAAGCTTGTTCTATTTCAGAGGGCTGATCCCCTTGCTCCCTCAAGCTCCCAGTCAATGGGCTTGCCAGCACCCCGGCCCCCACCCATTTTGGCGCCAACAGCTAAGGGCACGCGCGACAGGAAGCACAGCCCGCGCCAAACGCTGCCCCGCGCATGCGCGCCCTGGCTGTCAGCGGCCGCAGGGCCACCCGGAGATACCCCCAATATATGCCTCGGCATCGGTCCCGCCCCTCACCCCCACCCTGCGTGGCACCTACCGCCTGCGGACATGGTCCGGGAGCGAGCCTGCCGGGGAGGCAAGCGCAGGCACTCGGGCTGGAGCTGTTCGCGCTGGCATCTTGCAGGTTGCAGACGACAGAACAGCTCTGAACGAGACCCCGGC(+cgg)TTTTTCGCGCGCGCGGAAACCAATTGGGAGGGGGCGGCGCAAGCGGAAGCAGCATGTACCACACAGGGCAAGAGAGTGGGGGAAGACCATGCTATGCCCGAAGGGAGTGCGCATGCGCGCAACGGAGGCACCCACACCTCCTGGCTATAGTGGGCTCGTCCGTGCTGCGACTGGCGCCACCCACCTCCCCCTTGCCTGAAGAGGTGGCTCGGCACTTGAGAGCAGGTAGTGCTGCTCTTGCAGAGGATGACTCTGGTTTGCTTGCCAGCACCCGCATCCAGCCGCTCCAAGCCCCTGTAATTCCACTTCCAGGGGGTCCTACAGCCTCTTCTGGCCTCTATGGGCATTGCACTCACATGCACAAACACAACTTTAAAAAAAAAAAAATC NGG 10 8 17 8 24 0 100 5 10
    240704092844_hof5 CCAAAGAGGGGAACCCCTGAGGGTGTCCCCAAGCTTGTTCTATTTCAGAGGGCTGATCCCCTTGCTCCCTCAAGCTCCCAGTCAATGGGCTTGCCAGCACCCCGGCCCCCACCCATTTTGGCGCCAACAGCTAAGGGCACGCGCGACAGGAAGCACAGCCCGCGCCAAACGCTGCCCCGCGCATGCGCGCCCTGGCTGTCAGCGGCCGCAGGGCCACCCGGAGATACCCCCAATATATGCCTCGGCATCGGTCCCGCCCCTCACCCCCACCCTGCGTGGCACCTACCGCCTGCGGACATGGTCCGGGAGCGAGCCTGCCGGGGAGGCAAGCGCAGGCACTCGGGCTGGAGCTGTTCGCGCTGGCATCTTGCAGGTTGCAGACGACAGAACAGCTCTGAACGAGACCCCGGCTTTTTCGCGCGCGCGGAAACCAATTGGGAGGGGGCGGCGCAAGCGGAAGCAGCATGTACCACAC(+ctt)AGGGCAAGAGAGTGGGGGAAGACCATGCTATGCCCGAAGGGAGTGCGCATGCGCGCAACGGAGGCACCCACACCTCCTGGCTATAGTGGGCTCGTCCGTGCTGCGACTGGCGCCACCCACCTCCCCCTTGCCTGAAGAGGTGGCTCGGCACTTGAGAGCAGGTAGTGCTGCTCTTGCAGAGGATGACTCTGGTTTGCTTGCCAGCACCCGCATCCAGCCGCTCCAAGCCCCTGTAATTCCACTTCCAGGGGGTCCTACAGCCTCTTCTGGCCTCTATGGGCATTGCACTCACATGCACAAACACAACTTTAAAAAAAAAAAAATC NGG 10 8 17 8 24 0 100 5 10
    240704092659_p6ex CCAAAGAGGGGAACCCCTGAGGGTGTCCCCAAGCTTGTTCTATTTCAGAGGGCTGATCCCCTTGCTCCCTCAAGCTCCCAGTCAATGGGCTTGCCAGCACCCCGGCCCCCACCCATTTTGGCGCCAACAGCTAAGGGCACGCGCGACAGGAAGCACAGCCCGCGCCAAACGCTGCCCCGCGCATGCGCGCCCTGGCTGTCAGCGGCCGCAGGGCCACCCGGAGATACCCCCAATATATGCCTCGGCATCGGTCCCGCCCCTCACCCCCACCCTGCGTGGCACCTACCGCCTGCGGACATGGTCCGGGAGCGAGCCTGCCGGGGAGGCAAGCGCAGGCACTCGGGCTGGAGCTGTTCGCGCTGGCATCTTGCAGGTTGCAGACGACAGAACAGCTCTGAACGAGACCCCGGCTTTTTCGCGCGCGCGGAAACCAATT(+ctt)GGGAGGGGGCGGCGCAAGCGGAAGCAGCATGTACCACACAGGGCAAGAGAGTGGGGGAAGACCATGCTATGCCCGAAGGGAGTGCGCATGCGCGCAACGGAGGCACCCACACCTCCTGGCTATAGTGGGCTCGTCCGTGCTGCGACTGGCGCCACCCACCTCCCCCTTGCCTGAAGAGGTGGCTCGGCACTTGAGAGCAGGTAGTGCTGCTCTTGCAGAGGATGACTCTGGTTTGCTTGCCAGCACCCGCATCCAGCCGCTCCAAGCCCCTGTAATTCCACTTCCAGGGGGTCCTACAGCCTCTTCTGGCCTCTATGGGCATTGCACTCACATGCACAAACACAACTTTAAAAAAAAAAAAATC NGG 10 8 17 8 24 0 100 5 10