Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613120051_3oxl GGAGAAACTGGGTTAAGGCCAGAGAAGTGCCG(+AGGACA)GATCCTGCCCCAGATTTTGACTTTAGAGCTC NGG 10 8 17 8 24 0 100 5 10
    240613120050_65h4 CATGGGAGCTCTAAAGTCAAAATCTGGGGCAG(+AGGACA)GATCCGGCACTTCTCTGGCCTTAACCCAGTT NGG 10 8 17 8 24 0 100 5 10
    240613120048_6rfv GCCCGAGGTTCTGGTGGCCCAGCAACTGCAGG(+AGGACA)GATCCATAAATCCAGAGGCCAGGATTCAGGC NGG 10 8 17 8 24 0 100 5 10
    240613120047_565p ACTGAGCCTGAATCCTGGCCTCTGGATTTATG(+AGGACA)GATCCCTGCAGTTGCTGGGCCACCAGAACCT NGG 10 8 17 8 24 0 100 5 10
    240613120046_kjbx ACAAGGGGAAGGAAGCCCAGGTAGAGGGTATG(+AGGACA)GATCCCTCGAACCTGTCAGCAGACGGAACAC NGG 10 8 17 8 24 0 100 5 10
    240613120044_wd9e ACCGCGTGTTCCGTCTGCTGACAGGTTCGAGG(+AGGACA)GATCCATACCCTCTACCTGGGCTTCCTTCCC NGG 10 8 17 8 24 0 100 5 10
    240613120042_kef9 ATTCCTGGCCCTAGAAAGGAGGGTCTGCCGAG(+AGGACA)GTACCAGGGGCCTGGCCTCACTGGCTGGACC NGG 10 8 17 8 24 0 100 5 10
    240613120039_ra09 GGTGTGGTCCAGCCAGTGAGGCCAGGCCCCTG(+AGGACA)GTACCTCGGCAGACCCTCCTTTCTAGGGCCA NGG 10 8 17 8 24 0 100 5 10
    240613120037_out4 GCAGTGGGGGCTTTCTCAGGAGAGATGCCAGG(+AGGACA)GATCCCCAGGGACTGCACACCCCACTATCTC NGG 10 8 17 8 24 0 100 5 10
    240613120034_z69g TCTAGGAGATAGTGGGGTGTGCAGTCCCTGGG(+AGGACA)GATCCCTGGCATCTCTCCTGAGAAAGCCCCC NGG 10 8 17 8 24 0 100 5 10