Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613120146_ukti GCAGGGCAATGGCACGGCTGTGAGGTTGCGGG(+AGGACA)GTACCCAGGAGTTTCTTAGCAAAGATGCTTC NGG 10 8 17 8 24 0 100 5 10
    240613120144_8ew2 GTCTAGAAGCATCTTTGCTAAGAAACTCCTGG(+AGGACA)GTACCCCGCAACCTCACAGCCGTGCCATTGC NGG 10 8 17 8 24 0 100 5 10
    240613120142_wly3 GACGTTCAGGGTTTGCCCTCTTCTCCTCCTGG(+AGGACA)GTACCTTCCACTGCGCTCTTCACCCCCGCTG NGG 10 8 17 8 24 0 100 5 10
    240613120140_r7xo GCCTTCAGCGGGGGTGAAGAGCGCAGTGGAAG(+AGGACA)GTACCCAGGAGGAGAAGAGGGCAAACCCTGA NGG 10 8 17 8 24 0 100 5 10
    240613120138_em5k TCCTTGCATGTTTATGCTCTTATGTATTCCTG(+AGGACA)GTACCCAGGGAGGCCAGAAGAGGGCTGATTC NGG 10 8 17 8 24 0 100 5 10
    240613120135_xs0f CCAGGGAATCAGCCCTCTTCTGGCCTCCCTGG(+AGGACA)GTACCAGGAATACATAAGAGCATAAACATGC NGG 10 8 17 8 24 0 100 5 10
    240613120133_rv7g TGCTGCCGTGTATGTGCTGGGAATTGAACCAG(+AGGACA)GATCCTCTGGATTTGCAGCTAGAACTTTTAA NGG 10 8 17 8 24 0 100 5 10
    240613120131_g2zj CATGGTTAAAAGTTCTAGCTGCAAATCCAGAG(+AGGACA)GATCCTGGTTCAATTCCCAGCACATACACGG NGG 10 8 17 8 24 0 100 5 10
    240613120130_dcsx CAGCTTACTGTTGCAGCTGCCTGAGCAGCCTG(+AGGACA)GATCCTGCCTGAGGTTAGACCAGAGACCTCC NGG 10 8 17 8 24 0 100 5 10
    240613120128_3wu2 GAGGAGGAGGTCTCTGGTCTAACCTCAGGCAG(+AGGACA)GATCCAGGCTGCTCAGGCAGCTGCAACAGTA NGG 10 8 17 8 24 0 100 5 10