Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613120249_5tl8 ATTAGTTCTGATTCATCCCCGAGGGAAGAGAG(+AGGACA)GTACCCATGGCTTCAGTGAGCACATGCCAAG NGG 10 8 17 8 24 0 100 5 10
    240613120247_qw4p CTGGGCTTGGCATGTGCTCACTGAAGCCATGG(+AGGACA)GTACCTCTCTTCCCTCGGGGATGAATCAGAA NGG 10 8 17 8 24 0 100 5 10
    240613120245_wr4f TGGCAAGTGGCCAGCTGAGCCCAGGTTACTTG(+AGGACA)GTACCCACTCTGCCTTGAGCCTTCCTTCCCC NGG 10 8 17 8 24 0 100 5 10
    240613120244_57h0 CCCTGGGGGAAGGAAGGCTCAAGGCAGAGTGG(+AGGACA)GTACCAAGTAACCTGGGCTCAGCTGGCCACT NGG 10 8 17 8 24 0 100 5 10
    240613120242_uly6 CCGCTGAGATCCAGCATCCAGCTGTGGCCAGG(+AGGACA)GATCCACCACCTCTGTGTACACCCTCACCCG NGG 10 8 17 8 24 0 100 5 10
    240613120240_t1oo AGCAGCGGGTGAGGGTGTACACAGAGGTGGTG(+AGGACA)GATCCCTGGCCACAGCTGGATGCTGGATCTC NGG 10 8 17 8 24 0 100 5 10
    240613120239_r4iv GCTAAAAATTCTGCCTCTCTGTCACTGTGCTG(+AGGACA)GATCCGTCTAAAGTGGGACAGTTCAATGGTC NGG 10 8 17 8 24 0 100 5 10
    240613120238_asdl TTTCAGACCATTGAACTGTCCCACTTTAGACG(+AGGACA)GATCCAGCACAGTGACAGAGAGGCAGAATTT NGG 10 8 17 8 24 0 100 5 10
    240613120236_3prb ACCCAGTCTAAGCCCAGAGCTTGTTTGGGAGG(+AGGACA)GATCCTCTCCTTCCTCCTGCTTGATCCTGTA NGG 10 8 17 8 24 0 100 5 10
    240613120235_p52o GCTCCTACAGGATCAAGCAGGAGGAAGGAGAG(+AGGACA)GATCCCTCCCAAACAAGCTCTGGGCTTAGAC NGG 10 8 17 8 24 0 100 5 10