Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613120233_p42u CTTGATGTGTGAGGGGTAATACCCAGAGTGGG(+AGGACA)GTACCTGCTTCACAAAGGATAAGGGGAGGGT NGG 10 8 17 8 24 0 100 5 10
    240613120231_qd4a ATTCCACCCTCCCCTTATCCTTTGTGAAGCAG(+AGGACA)GTACCCCACTCTGGGTATTACCCCTCACACA NGG 10 8 17 8 24 0 100 5 10
    240613120230_6nv6 TGCCTGCCTGCCTGCCTGCCTGCCTACCTGTG(+AGGACA)GATCCTGTTCCCCTAAATGGTCTGCCTTGTC NGG 10 8 17 8 24 0 100 5 10
    240613120228_bu9q GGCCAGACAAGGCAGACCATTTAGGGGAACAG(+AGGACA)GATCCACAGGTAGGCAGGCAGGCAGGCAGGC NGG 10 8 17 8 24 0 100 5 10
    240613120227_ajxy AGGAAGGCAGGAGGGGTGCCCGTCCCGTTGGG(+AGGACA)GATCCTAGCACAAGGCCTGACCAGGTCCCTG NGG 10 8 17 8 24 0 100 5 10
    240613120226_6uty AAGTCCAGGGACCTGGTCAGGCCTTGTGCTAG(+AGGACA)GATCCCCAACGGGACGGGCACCCCTCCTGCC NGG 10 8 17 8 24 0 100 5 10
    240613120225_lsdl AAATAGGAATTTACATAGGAATGTGAGATGTG(+AGGACA)GTACCTGCCTTTTAGGGAGAGATCAAAACTA NGG 10 8 17 8 24 0 100 5 10
    240613120223_a1le TAGTTTAGTTTTGATCTCTCCCTAAAAGGCAG(+AGGACA)GTACCACATCTCACATTCCTATGTAAATTCC NGG 10 8 17 8 24 0 100 5 10
    240613120222_dvmr GACAGAGGTGTGGTGTGGTGTCAGGGTGCTGG(+AGGACA)GTACCTTGCCTTTTCTGCCCAGGGCTTGCTC NGG 10 8 17 8 24 0 100 5 10
    240613120220_jw9q AAGGAGAGCAAGCCCTGGGCAGAAAAGGCAAG(+AGGACA)GTACCCAGCACCCTGACACCACACCACACCT NGG 10 8 17 8 24 0 100 5 10